PMID- 15910622
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20220330
IS  - 0742-3071 (Print)
IS  - 0742-3071 (Linking)
VI  - 22
IP  - 6
DP  - 2005 Jun
TI  - Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid 
      of patients with proliferative diabetic retinopathy.
PG  - 719-22
AB  - AIMS: To determine the intra-vitreous levels of two pro-inflammatory cytokines 
      [interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1)] and the 
      anti-inflammatory cytokine interleukin-10 (IL-10) in patients with proliferative 
      diabetic retinopathy (PDR). In addition, the relationship between the profile of 
      cytokines and PDR activity has also been evaluated. PATIENTS AND METHODS: The 
      study included 22 consecutive diabetic patients with PDR (4 Type 1 and 18 Type 2) 
      on whom a vitrectomy was performed. Sixteen age-matched non-diabetic patients 
      with other conditions requiring vitrectomy, but in which the retina was not 
      directly affected by neovascularization served as a control group. IL-8, MCP-1 
      and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: 
      The vitreal levels of both IL-8 and MCP-1 were strikingly higher in diabetic 
      patients with PDR in comparison with the control group [173.5 (64-1670) vs. 49 
      pg/ml (25-145), P < 0.001, and 2171 (388-6155) vs. 438 pg/ml (207-1344), P < 
      0.001, respectively]. In addition, the vitreous concentrations of IL-8 and MCP-1 
      were higher in patients with active PDR than in those patients with quiescent PDR 
      [324.5 (80-1670) vs. 173.5 pg/ml (64-487), P = 0.06 and 3596 (1670-6155) vs. 1143 
      pg/ml (388-2500), P = 0.01, respectively]. However, vitreal levels of IL-10 in 
      diabetic patients were similar to that obtained in the control group [2.89 
      (1.55-5.50) vs. 2.46 pg/ml (2.2-5.41), P = NS]. CONCLUSIONS: The pro-inflammatory 
      cytokines IL-8 and MCP-1 are increased in the vitreous fluid of PDR patients 
      without an increase in the anti-inflammatory cytokine IL-10. In addition, both 
      IL-8 and MCP-1 intra-vitreous levels correlated with PDR activity, thus 
      suggesting that these cytokines may be pathogenically important in PDR.
FAU - Hernandez, C
AU  - Hernandez C
AD  - Diabetes Research Unit (Division of Endocrinology), Hospital Universitari Vall 
      d'Hebron, Barcelona, Spain.
FAU - Segura, R M
AU  - Segura RM
FAU - Fonollosa, A
AU  - Fonollosa A
FAU - Carrasco, E
AU  - Carrasco E
FAU - Francisco, G
AU  - Francisco G
FAU - Simo, R
AU  - Simo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Diabet Med
JT  - Diabetic medicine : a journal of the British Diabetic Association
JID - 8500858
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Aged
MH  - Chemokine CCL2/*metabolism
MH  - Diabetes Mellitus, Type 1/metabolism
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Diabetic Retinopathy/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Humans
MH  - Interleukin-10/*metabolism
MH  - Interleukin-8/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Vitreous Body/*chemistry
EDAT- 2005/05/25 09:00
MHDA- 2005/08/02 09:00
CRDT- 2005/05/25 09:00
PHST- 2005/05/25 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2005/05/25 09:00 [entrez]
AID - DME1538 [pii]
AID - 10.1111/j.1464-5491.2005.01538.x [doi]
PST - ppublish
SO  - Diabet Med. 2005 Jun;22(6):719-22. doi: 10.1111/j.1464-5491.2005.01538.x.