PMID- 15911540
OWN - NLM
STAT- MEDLINE
DCOM- 20050929
LR  - 20181113
IS  - 0305-7364 (Print)
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Linking)
VI  - 96
IP  - 2
DP  - 2005 Aug
TI  - Localization of the 5S and 45S rDNA sites and cpDNA sequence analysis in species 
      of the Quadrifaria group of Paspalum (Poaceae, Paniceae).
PG  - 191-200
AB  - BACKGROUND AND AIMS: The Quadrifaria group of Paspalum (Poaceae, Paniceae) 
      comprises species native to the subtropical and temperate regions of South 
      America. The purpose of this research was to characterize the I genomes in five 
      species of this group and to establish phylogenetic relationships among them. 
      METHODS: Prometaphase chromatin condensation patterns, the physical location of 
      5S and 45S rDNA sites by fluorescence in situ hybridization (FISH), and sequences 
      of five chloroplast non-coding regions were analysed. KEY RESULTS: The 
      condensation patterns observed were highly conserved among diploid and tetraploid 
      accessions studied and not influenced by the dyes used or by the FISH procedure, 
      allowing the identification of almost all the chromosome pairs that carried the 
      rDNA signals. The FISH analysis of 5S rDNA sites showed the same localization and 
      a correspondence between the number of sites and ploidy level. In contrast, the 
      distribution of 45S rDNA sites was variable. Two general patterns were observed 
      with respect to the location of the 45S rDNA. The species and cytotypes Paspalum 
      haumanii 2x, P. intermedium 2x, P. quadrifarium 4x and P. exaltatum 4x showed 
      proximal sites on chromosome 8 and two to four distal sites in other chromosomes, 
      while P. quarinii 4x and P. quadrifarium 2x showed only distal sites located on a 
      variable number of small chromosomes and on the long arm of chromosome 1. The 
      single most-parsimonious tree found from the phylogenetic analysis showed the 
      Quadrifaria species partitioned in two clades, one of them includes P. haumanii 
      2x and P. intermedium 2x together with P. quadrifarium 4x and P. exaltatum 4x, 
      while the other contains P. quadrifarium 2x and P. quarinii 4x. CONCLUSIONS: The 
      subdivision found with FISH is consistent with the clades recovered with cpDNA 
      data and both analyses suggest that the Quadrifaria group, as presently defined, 
      is not monophyletic and its species belong in at least two clades.
FAU - Vaio, Magdalena
AU  - Vaio M
AD  - Laboratory of Genetics, Department of Plant Biology, Facultad de Agronomia, 
      Universidad de la Republica, Av. E. Garzon 780 CP12900, Montevideo, Uruguay.
FAU - Speranza, Pablo
AU  - Speranza P
FAU - Valls, Jose Francisco
AU  - Valls JF
FAU - Guerra, Marcelo
AU  - Guerra M
FAU - Mazzella, Cristina
AU  - Mazzella C
LA  - eng
SI  - GENBANK/AY941120
SI  - GENBANK/AY941121
SI  - GENBANK/AY941122
SI  - GENBANK/AY941123
SI  - GENBANK/AY941124
SI  - GENBANK/AY941125
SI  - GENBANK/AY941126
SI  - GENBANK/AY941127
SI  - GENBANK/AY941128
SI  - GENBANK/AY941129
SI  - GENBANK/AY941130
SI  - GENBANK/AY941131
SI  - GENBANK/AY941132
SI  - GENBANK/AY941133
SI  - GENBANK/AY941134
SI  - GENBANK/AY941135
SI  - GENBANK/AY941136
SI  - GENBANK/AY941137
SI  - GENBANK/AY941138
SI  - GENBANK/AY941139
SI  - GENBANK/AY941140
SI  - GENBANK/AY941141
SI  - GENBANK/AY941142
SI  - GENBANK/AY941143
SI  - GENBANK/AY941144
SI  - GENBANK/AY941145
SI  - GENBANK/AY941146
SI  - GENBANK/AY941147
SI  - GENBANK/AY941148
SI  - GENBANK/AY941149
SI  - GENBANK/AY941150
SI  - GENBANK/AY941151
SI  - GENBANK/AY941152
SI  - GENBANK/AY941153
SI  - GENBANK/AY941154
SI  - GENBANK/AY941155
SI  - GENBANK/AY941156
SI  - GENBANK/AY941157
SI  - GENBANK/AY941158
SI  - GENBANK/AY941159
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050523
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 0 (DNA, Chloroplast)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - DNA, Chloroplast/chemistry/*genetics
MH  - DNA, Ribosomal/genetics
MH  - Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Paspalum/*genetics
MH  - Phylogeny
MH  - Polyploidy
MH  - Prometaphase/genetics
MH  - RNA, Ribosomal/*genetics
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
PMC - PMC4246868
EDAT- 2005/05/25 09:00
MHDA- 2005/09/30 09:00
PMCR- 2006/08/01
CRDT- 2005/05/25 09:00
PHST- 2005/05/25 09:00 [pubmed]
PHST- 2005/09/30 09:00 [medline]
PHST- 2005/05/25 09:00 [entrez]
PHST- 2006/08/01 00:00 [pmc-release]
AID - mci168 [pii]
AID - 10.1093/aob/mci168 [doi]
PST - ppublish
SO  - Ann Bot. 2005 Aug;96(2):191-200. doi: 10.1093/aob/mci168. Epub 2005 May 23.