PMID- 15911583
OWN - NLM
STAT- MEDLINE
DCOM- 20060210
LR  - 20240324
IS  - 0016-6731 (Print)
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Linking)
VI  - 170
IP  - 3
DP  - 2005 Jul
TI  - Rapid and repeatable elimination of a parental genome-specific DNA repeat 
      (pGc1R-1a) in newly synthesized wheat allopolyploids.
PG  - 1239-45
AB  - Recent work in the Triticum-Aegilops complex demonstrates that 
      allopolyploidization is associated with an array of changes in low-copy coding 
      and noncoding sequences. Nevertheless, the behavior and fate of repetitive DNA 
      elements that constitute the bulk of nuclear DNA of these plant species is less 
      clear following allopolyploidy. To gain further insight into the genomic events 
      that accompany allopolyploid formation, we investigated fluorescence in situ 
      hybridization (FISH) patterns of a parental-specific, tandem DNA repeat (pGc1R-1) 
      on three sets of newly synthesized amphiploids with different parental species. 
      It was found that drastic physical elimination of pGc1R-1 copies occurred in all 
      three amphiploids in early generations. DNA gel-blot analysis confirmed the FISH 
      data and estimates indicated that approximately 70-90% of the copies of the 
      pGc1R-1 repeat family were eliminated from the amphiploids by the second to third 
      selfed generations. Thus, allopolyploidy in Triticum-Aegilops can be accompanied 
      by rapid and extensive elimination of parental-specific repetitive DNA sequences, 
      which presumably play a role in the initial stabilization of the nascent 
      amphiploid plants.
FAU - Han, Fangpu
AU  - Han F
AD  - Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, 
      Northeast Normal University, Changchun 130024, China.
FAU - Fedak, George
AU  - Fedak G
FAU - Guo, Wanli
AU  - Guo W
FAU - Liu, Bao
AU  - Liu B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050523
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - Electrophoresis
MH  - In Situ Hybridization, Fluorescence
MH  - *Polyploidy
MH  - Sequence Deletion/*genetics
MH  - Tandem Repeat Sequences/*genetics
MH  - Triticum/*genetics
PMC - PMC1451191
EDAT- 2005/05/25 09:00
MHDA- 2006/02/14 09:00
PMCR- 2005/10/01
CRDT- 2005/05/25 09:00
PHST- 2005/05/25 09:00 [pubmed]
PHST- 2006/02/14 09:00 [medline]
PHST- 2005/05/25 09:00 [entrez]
PHST- 2005/10/01 00:00 [pmc-release]
AID - genetics.104.039263 [pii]
AID - 9263 [pii]
AID - 10.1534/genetics.104.039263 [doi]
PST - ppublish
SO  - Genetics. 2005 Jul;170(3):1239-45. doi: 10.1534/genetics.104.039263. Epub 2005 
      May 23.