PMID- 15916742
OWN - NLM
STAT- MEDLINE
DCOM- 20051207
LR  - 20140729
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 26
IP  - 6
DP  - 2005 Jun
TI  - Melatonin-selenium nanoparticles protects liver against immunological injury 
      induced by bacillus Calmette-Guerin and lipopolysaccharide.
PG  - 745-52
AB  - AIM: Melatonin-selenium nanoparticle (MT-Se), a novel complex, was synthesized by 
      preparing selenium nanoparticles in a melatonin medium. The present investigation 
      was designed to determine the protective effects of MT-Se against immunological 
      liver injury in mice induced by bacillus Calmette-Guerin (BCG)/lipopolysaccharide 
      (LPS). METHODS: The model of immunological liver injury in mice was prepared. The 
      levels of alanine aminotransferase, aspartate amino-transferase, nitric oxide 
      (NO) in serum, malondialdehyde content, superoxide dismutase (SOD), and 
      glutathione peroxidase (GSH-px) activities in a liver homogenate were assayed by 
      spectrophotometry. The content of tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-1 (IL-1) were determined by ELISA. The splenocyte proliferation was 
      assayed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) dye 
      reduction. Meanwhile, a hepatic pathological examination was observed. RESULTS: 
      In the BCG/LPS-induced hepatic injury model, MT-Se administered at doses of 5, 
      10, or 20 mg/kg to the BCG/LPS-treated mice for 10 d significantly reduced the 
      increase in serum aminotransferase, reduced the severe extent of hepatic cell 
      damage and the immigration of inflammatory cells. It also attenuated the increase 
      in the content of thiobarbituric acid-reactive substances and enhanced the 
      decrease in activities of SOD and GSH-px. In contrast, the treatment with MT-Se 
      suppressed the increase in NO level in both the serum and liver tissue. 
      Furthermore, MT-Se significantly lowered an increase in TNF-alpha and IL-1beta 
      levels in the liver and inhibited the production of TNF- alpha and IL-1beta by 
      peritoneal macrophages. A downregulation effect of MT-Se on splenocyte 
      proliferation was also observed. CONCLUSION: MT-Se showed a hepatic protective 
      action on immunological liver injury in mice.
FAU - Wang, Hua
AU  - Wang H
AD  - Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, 
      China.
FAU - Wei, Wei
AU  - Wei W
FAU - Zhang, Sheng-yi
AU  - Zhang SY
FAU - Shen, Yu-xian
AU  - Shen YX
FAU - Wang, Ni-ping
AU  - Wang NP
FAU - Yue, Li
AU  - Yue L
FAU - Xu, Shu-yun
AU  - Xu SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - H6241UJ22B (Selenium)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Glutathione Peroxidase/metabolism
MH  - *Hepatitis, Animal/chemically induced/metabolism
MH  - Interleukin-1/biosynthesis/metabolism
MH  - Lipopolysaccharides
MH  - Liver/metabolism/*pathology
MH  - Macrophages, Peritoneal/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Melatonin/*pharmacology
MH  - Mice
MH  - Mycobacterium bovis
MH  - Nanostructures
MH  - Nitric Oxide/blood/metabolism
MH  - Selenium/*pharmacology
MH  - Superoxide Dismutase/metabolism
MH  - Transaminases/blood
MH  - Tumor Necrosis Factor-alpha/biosynthesis/metabolism
EDAT- 2005/05/27 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/05/27 09:00
PHST- 2005/05/27 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/05/27 09:00 [entrez]
AID - 10.1111/j.1745-7254.2005.00745.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2005 Jun;26(6):745-52. doi: 10.1111/j.1745-7254.2005.00745.x.