PMID- 15917696
OWN - NLM
STAT- MEDLINE
DCOM- 20050914
LR  - 20191026
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 15
IP  - 3
DP  - 2005 Jun
TI  - Fluorescence in situ hybridization (FISH) evaluation of chromosomes 6, 7, 9 and 
      10 throughout human melanocytic tumorigenesis.
PG  - 155-60
AB  - Loss of the 9p21 region, 6q and 10q and gain of chromosome 7 are the most 
      frequent chromosomal abnormalities found in human melanomas, but very few 
      cytogenetic data are available regarding dysplastic and common naevi. To study 
      the occurrence of the most consistent chromosomal changes during melanocytic 
      tumorigenesis, archival samples from 30 common naevi and 30 naevus-associated 
      melanomas were analysed by interphase fluorescence in situ hybridization (FISH) 
      using centromeric probes for chromosomes 9 and 7 and locus-specific probes for 
      9p21, 6q11.1, 6q24.1, 10p15.3 and 10q23.1 regions. In naevus-associated 
      melanomas, separate evaluations were made for sectors corresponding to residual 
      naevus, dysplastic naevus, radial growth phase melanoma and vertical growth phase 
      melanoma. No chromosomal aberrations were found in common naevi, but monosomy 7 
      was observed in one case. In naevus-associated melanomas, loss of the entire 
      chromosome 9 or of the 9p21 region was observed in 56% of common and 54% of 
      dysplastic naevus sectors, in 64% of radial growth phase melanoma and in 82% of 
      vertical growth phase melanoma. Loss of the long arm of chromosome 6, monosomy 10 
      and deletion 10q were exclusively confined to radial (18% for both chromosomes) 
      and vertical (29 and 59%, respectively) growth phase melanomas. Polysomy of 
      chromosome 7 was detected only in melanoma sectors (radial growth phase, 14%; 
      vertical growth phase, 59%). The high incidence of 9p21 loss in 
      melanoma-associated naevi, which is maintained in all evolutionary phases of 
      melanocytic tumorigenesis, and the complete absence of chromosomal aberrations in 
      common naevi, strongly suggest that 9p21 loss may be regarded as a cytogenetic 
      marker of melanocytic naevi with a high potential for progression.
FAU - Casorzo, Laura
AU  - Casorzo L
AD  - Unit of Pathology, Institute for Cancer Research and Treatment, Strada 
      Provinciale 142, 10060 Candiolo-Torino, Italy.
FAU - Luzzi, Carmen
AU  - Luzzi C
FAU - Nardacchione, Antonella
AU  - Nardacchione A
FAU - Picciotto, Franco
AU  - Picciotto F
FAU - Pisacane, Alberto
AU  - Pisacane A
FAU - Risio, Mauro
AU  - Risio M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 10
MH  - Chromosomes, Human, Pair 6
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 9
MH  - DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/chemistry/*genetics
MH  - Monosomy/genetics
MH  - Nevus, Pigmented/chemistry/*genetics
MH  - Precancerous Conditions/chemistry/*genetics
MH  - Skin Neoplasms/chemistry/*genetics
EDAT- 2005/05/27 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/05/27 09:00
PHST- 2005/05/27 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/05/27 09:00 [entrez]
AID - 00008390-200506000-00003 [pii]
AID - 10.1097/00008390-200506000-00003 [doi]
PST - ppublish
SO  - Melanoma Res. 2005 Jun;15(3):155-60. doi: 10.1097/00008390-200506000-00003.