PMID- 15919206
OWN - NLM
STAT- MEDLINE
DCOM- 20060913
LR  - 20061115
IS  - 0960-8524 (Print)
IS  - 0960-8524 (Linking)
VI  - 97
IP  - 4
DP  - 2006 Mar
TI  - Comparison of long-term performances and final microbial compositions of 
      anaerobic reactors treating landfill leachate.
PG  - 641-7
AB  - Laboratory scale anaerobic upflow filter, sludge blanket and hybrid bed reactors 
      were operated for 860 days in the treatment of high ammonia landfill leachate. 
      Organic loading was gradually increased from 1.3 to 23.5 kg COD/m3 day in the 
      start-up period and then fluctuated according to the COD concentration of raw 
      leachate. To prevent free ammonia inhibition, influent pH was reduced to 4.5 
      after Day 181 and consequently COD removal efficiencies above 80% were achieved 
      in all reactors. However, the anaerobic filter and hybrid bed reactor were 
      generally found slightly more efficient and stable than the UASB reactor. In 
      addition to conventional anaerobic reactor control parameters, the complementary 
      techniques of denaturing gradient gel electrophoresis (DGGE), cloning and 
      fluorescent in situ hybridization (FISH) were used to identify and compare the 
      microbial profiles in the reactors at Day 830. Molecular analyses revealed that 
      acetoclastic Methanosaeta species were prevalent in all reactors and 
      configuration did not have an impact on microbial diversity in the long-term.
FAU - Calli, B
AU  - Calli B
AD  - Department of Environmental Engineering, Marmara University, 34722 Goztepe, 
      Istanbul, Turkey. bcalli@eng.marmara.tr
FAU - Mertoglu, B
AU  - Mertoglu B
FAU - Roest, K
AU  - Roest K
FAU - Inanc, B
AU  - Inanc B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (DNA Primers)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/metabolism
MH  - Bacteria, Anaerobic/genetics/*metabolism
MH  - *Biodiversity
MH  - *Bioreactors
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Electrophoresis
MH  - In Situ Hybridization, Fluorescence
MH  - Waste Disposal, Fluid/*methods
MH  - Water Purification/*methods
EDAT- 2005/05/28 09:00
MHDA- 2006/09/14 09:00
CRDT- 2005/05/28 09:00
PHST- 2004/03/16 00:00 [received]
PHST- 2004/11/26 00:00 [revised]
PHST- 2005/03/22 00:00 [accepted]
PHST- 2005/05/28 09:00 [pubmed]
PHST- 2006/09/14 09:00 [medline]
PHST- 2005/05/28 09:00 [entrez]
AID - S0960-8524(05)00184-7 [pii]
AID - 10.1016/j.biortech.2005.03.021 [doi]
PST - ppublish
SO  - Bioresour Technol. 2006 Mar;97(4):641-7. doi: 10.1016/j.biortech.2005.03.021.