PMID- 1592090
OWN - NLM
STAT- MEDLINE
DCOM- 19920702
LR  - 20190824
IS  - 0014-4894 (Print)
IS  - 0014-4894 (Linking)
VI  - 74
IP  - 4
DP  - 1992 Jun
TI  - Experimental onchocerciasis in chimpanzees. Antibody response and antigen 
      recognition after primary infection with Onchocerca volvulus.
PG  - 367-80
AB  - Nine of 18 chimpanzees inoculated with 250 infective third-stage larvae (L3) each 
      developed patent (i.e., positive for microfilariae) Onchocerca volvulus 
      infection. Four of 6 infected chimpanzees that received 200 micrograms/kg 
      ivermectin at 28 days postinfection (pi) became patent, whereas, when ivermectin 
      was given concurrently with L3 challenge only 1 of 6 infected animals developed 
      patent infection. The antibody response to O. volvulus adult worm-derived 
      antigens (OvAg) showed clear differences between patent and nonpatent 
      chimpanzees. Three months pi, all sera detected several OvAg in the range of M(r) 
      35-120 k. Sera collected 6 mo pi from later patent animals recognized increasing 
      numbers of OvAg, especially in the lower MW range of M(r) 13 to 33 k. Beginning 
      10 months pi Onchocerca-antigens of M(r) 21, 24, 26, and 28 k were detected only 
      by patent chimpanzee's sera. The antibody response in nonpatent chimpanzees 
      consistently recognized fewer OvAg, most of which were limited to the higher M(r) 
      range (35-120 k). The reactivity of sera from infected chimpanzees to a low 
      molecular weight fraction (LMW) of total OvAg doubled within 6 months pi, and 
      increased continuously in patent animals from 13 until 30 months pi. Serological 
      reactivity of nonpatent animals to LMW-OvAg remained low. The titers of 
      circulating IgG directed against total OvAg increased in all infected 
      chimpanzees, and continued to rise with patency. In nonpatent chimpanzees the 
      antibody production gradually returned to preinfection values. Total and 
      OvAg-specific IgE increased in patent and nonpatent chimpanzees. Also, during 
      prepatency the granulocyte and antibody-mediated in vitro killing of 
      microfilariae of O. volvulus increased in subsequently patent chimpanzees. The in 
      vitro immobilization of L3 remained low.
FAU - Soboslay, P T
AU  - Soboslay PT
AD  - Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
FAU - Weiss, N
AU  - Weiss N
FAU - Dreweck, C M
AU  - Dreweck CM
FAU - Taylor, H R
AU  - Taylor HR
FAU - Brotman, B
AU  - Brotman B
FAU - Schulz-Key, H
AU  - Schulz-Key H
FAU - Greene, B M
AU  - Greene BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 70288-86-7 (Ivermectin)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*biosynthesis/blood/immunology
MH  - Antigens, Helminth/*immunology
MH  - Granulocytes/immunology
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Ivermectin/pharmacology
MH  - Microfilariae/immunology
MH  - Molecular Weight
MH  - Onchocerca/growth & development/*immunology
MH  - Onchocerciasis/*immunology/parasitology
MH  - Pan troglodytes
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1016/0014-4894(92)90199-k [doi]
PST - ppublish
SO  - Exp Parasitol. 1992 Jun;74(4):367-80. doi: 10.1016/0014-4894(92)90199-k.