PMID- 15921626
OWN - NLM
STAT- MEDLINE
DCOM- 20090209
LR  - 20050530
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 26
IP  - 2
DP  - 2005 Feb
TI  - [Establishment and characterization of a human acute monocytic leukemic cell 
      line, SHI-1, carrying t(6;11)(q27;23) and p53 gene alteration].
PG  - 94-9
AB  - OBJECTIVE: To establish a novel human monocytic leukemic cell line and 
      characterize its biological features. METHODS: Mononuclear cells isolated from 
      the bone marrow of an acute monocytic leukemia (AML-M(5b)) patient at relapse 
      were inoculated in a liquid culture system. And the biologic features of the 
      established cell line SHI-1 were characterized by morphology, cytochemical 
      staining, flow cytometry, karyotypic analysis, polymerase chain reaction (PCR), 
      reverse transcriptase PCR (RT-PCR), fluorescence in situ hybridization (FISH), 
      tumorigenicity in nude mice, quantitative fluorescent polymerase chain reaction, 
      broth medium culture, short tandem repeating sequences-PCR (STR-PCR), 
      multiplex-FISH (M-FISH), and (3)H-thymidine incorporation assay. RESULTS: A human 
      acute monocytic leukemia cell line, SHI-1, was established and has proliferated 
      continuously in vitro for over one year. The cell line presented typical 
      morphology and immuno-profile of monocytic lineage with the original 
      t(6;11)(q27;q23) and del(17)(p11) abnormalities. The MLL-AF6 fusion transcript 
      was detected by RT-PCR. The rearrangement of MLL gene, deletion of p53 gene, and 
      translocation between chromosomes 6 and 11 were revealed by FISH. A point 
      mutation of ATC-->ACC at exon 6 of the p53 gene was found by sequencing of the 
      PCR products. The clonality and the high tumorigenicity of the SHI-1 cell line 
      were confirmed. Infections of EBV and mycoplasma were excluded. A derivative 
      chromosome 7 resulting from a translocation between chromosomes 7 and 13, 
      monosomy 18 and a minute derived from chromosome 8 in addition to t(6;11) and 
      deletion(17)(p11) were detected by M-FISH in SHI-1 cells passaged to March 2003. 
      Cell line authentication by STR-PCR confirmed the identity to the original 
      leukemic cells of the patient. (3)H-thymidine incorporation assay showed that 
      IL-4 and IL-15 had proliferative effects, while IFN-gamma, TNFalpha, IL-2, PDGF, 
      and IL-7 had inhibitory effects on the cell line. CONCLUSIONS: SHI-1 is a novel 
      acute monocytic leukemia-derived cell line carrying t(6;11)(q27;q23) and p53 gene 
      alteration and having high tumorigenicity in nude mice. It provides a new useful 
      tool for leukemia research.
FAU - Chen, Su-ning
AU  - Chen SN
AD  - First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, 
      Suzhou 215006, China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Zhang, Xue-guang
AU  - Zhang XG
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Wang, Yong
AU  - Wang Y
FAU - Cen, Jian-nong
AU  - Cen JN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Bone Marrow Cells/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Experimental/blood/genetics/pathology
MH  - Leukemia, Monocytic, Acute/blood/*genetics/pathology
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - *Translocation, Genetic
MH  - Transplantation, Heterologous
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2005/06/01 09:00
MHDA- 2009/02/10 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2009/02/10 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2005 Feb;26(2):94-9.