PMID- 15922722
OWN - NLM
STAT- MEDLINE
DCOM- 20050811
LR  - 20181201
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 232
IP  - 1-2
DP  - 2004 Nov-Dec
TI  - Dexamethasone selectively inhibits differentiation of cord blood stem cell 
      derived-dendritic cell (DC) precursors into immature DCs.
PG  - 127-36
AB  - Perinatal dexamethasone (Dx) alters the immune system leading to increased 
      infections and developmental abnormalities. Dendritic cells (DCs) derived from 
      cord-blood monocytes are especially Dx sensitive and we sought to determine the 
      effects of Dx on cord-blood CD34+-DCs. Distinct stages of cord-blood CD34+-DC 
      development were delineated: pre-DC, immature, and mature DCs. Dx added during 
      development of pre-DCs did not suppress precursor number, or translocate the 
      glucocorticoid receptor (GcR) from the cytoplasm to the nucleus. However, Dx 
      added during pre-DCs differentiation into immature DCs, prompted GcR 
      translocation to the nucleus, enhanced DC apoptosis, suppressed differentiation 
      to CD1a+ cells, inhibited expression of CD86, reduced subsequent CD83 expression, 
      maintained DC endocytic activity, suppressed IL-6 secretion, enhanced IL-10 
      secretion, and reduced DC-mediated T cell stimulation. Dx added during the 
      maturation stage caused less dramatic effects. Thus, Dx stalled maturation, 
      selectively induced apoptosis of developing DCs and the sensitivity peaked during 
      pre-DCs differentiation into immature DCs.
FAU - Mainali, Elsie S
AU  - Mainali ES
AD  - Department of Pediatrics, Virginia Commonwealth University Health System, 401 
      North 12th Street, Room 6-500, Richmond, VA 23298, USA. emainali@hsc.vcu.edu
FAU - Tew, John G
AU  - Tew JG
LA  - eng
GR  - P30 CA16059/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050426
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, CD34)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Antigens, CD34/biosynthesis/immunology
MH  - Apoptosis/drug effects
MH  - Cell Differentiation/*drug effects/immunology
MH  - Cytokines/drug effects/immunology/metabolism
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Dexamethasone/*pharmacology
MH  - Fetal Blood/*cytology/immunology
MH  - Humans
MH  - Phenotype
MH  - Receptors, Glucocorticoid/drug effects/metabolism
MH  - Stem Cells/*drug effects/immunology
MH  - T-Lymphocytes/drug effects/physiology
EDAT- 2005/06/01 09:00
MHDA- 2005/08/12 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/01/20 00:00 [received]
PHST- 2005/03/21 00:00 [revised]
PHST- 2005/03/22 00:00 [accepted]
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2005/08/12 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - S0008-8749(05)00042-0 [pii]
AID - 10.1016/j.cellimm.2005.03.002 [doi]
PST - ppublish
SO  - Cell Immunol. 2004 Nov-Dec;232(1-2):127-36. doi: 10.1016/j.cellimm.2005.03.002. 
      Epub 2005 Apr 26.