PMID- 15923358
OWN - NLM
STAT- MEDLINE
DCOM- 20051019
LR  - 20061115
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 10
DP  - 2005 Oct
TI  - Increased efficiency of fluorescence in situ hybridization (FISH) using the 
      microwave.
PG  - 1301-3
AB  - A new method is described for performing fluorescence in situ hybridization 
      (FISH). FISH signals are enhanced by microwave pulses applied during the DNA-DNA 
      hybridization process. It is the first description of FISH with a single/low-copy 
      probe done more efficiently by application of microwave; the latter leads to 
      quick results or enhancement of weak signals. Microwave FISH has been compared 
      systematically with normal FISH, and we could demonstrate the efficiency of 
      microwave irradiation especially in the first 100 min of hybridization.
FAU - Weise, Anja
AU  - Weise A
AD  - Institut fur Humangenetik und Anthropologie, Jena, Germany.
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Claussen, Uwe
AU  - Claussen U
FAU - Halbhuber, Karl-Jurgen
AU  - Halbhuber KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050527
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - DNA/chemistry
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Microwaves
MH  - Prenatal Diagnosis/methods
EDAT- 2005/06/01 09:00
MHDA- 2005/10/20 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2005/10/20 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - jhc.5B6632.2005 [pii]
AID - 10.1369/jhc.5B6632.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Oct;53(10):1301-3. doi: 10.1369/jhc.5B6632.2005. Epub 
      2005 May 27.