PMID- 15924277
OWN - NLM
STAT- MEDLINE
DCOM- 20061108
LR  - 20181113
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 262
IP  - 11
DP  - 2005 Nov
TI  - Immunohistochemical demonstration of multiple HPV types in laryngeal squamous 
      cell carcinoma.
PG  - 917-20
AB  - The aim of this study was to determine the prevalence of human papillomaviruses 
      (HPV) types 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58 in laryngeal squamous 
      cell carcinoma specimens using immunohistochemical reactions and to correlate the 
      presence of HPV with the clinical and pathological characteristics of these 
      patients. Tissue samples were collected from 40 patients with primary laryngeal 
      squamous cell carcinoma (LSCC) and from 33 subjects with non-neoplastic laryngeal 
      lesions or laryngeal nodules, which served as a control group. Human papilloma 
      virus was detected in 6 (15%) of the 40 patients. Five (83.4%) of six patients 
      with HPV positive tumors had G2 (moderately differentiated), one patient (16.6%) 
      had G3 (poorly differentiated), and no patient with HPV positive tumor had a G1 
      (well-differentiated) tumor. Four (66.6%) of the six HPV positive tumors were in 
      the supraglottic region, one (16.6%) tumor was located in the glottis, and one 
      (16.6%) HPV positive tumor was in the subglotic region. Five (83.4%) of six HPV 
      positive tumors were T3-T4, and one was T2. Three of six HPV positive patients 
      had no clinically evident cervical lymph nodes (N0), and three of the HPV 
      positive patients were N1 or N2. Human papillomavirus was not detected in any of 
      the samples from the control group. The presence of HPV infection in 15% of the 
      cases may suggest a possible role in the etiology of laryngeal squamous cell 
      carcinoma. However, no significant correlation between HPV incidence and 
      histological grading and clinical staging could be demonstrated.
FAU - Morshed, Kamal
AU  - Morshed K
AD  - Department of Otolaryngology Head and Neck Surgery, Medical University of Lublin, 
      Poland. kamal1@op.pl
FAU - Korobowicz, Elzbieta
AU  - Korobowicz E
FAU - Szymanski, Marcin
AU  - Szymanski M
FAU - Skomra, Danuta
AU  - Skomra D
FAU - Golabek, Wieslaw
AU  - Golabek W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050529
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/pathology/*virology
MH  - DNA, Viral/genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/pathology/*virology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Papillomavirus Infections/*diagnosis
MH  - Polymerase Chain Reaction
EDAT- 2005/06/01 09:00
MHDA- 2006/11/10 09:00
CRDT- 2005/06/01 09:00
PHST- 2004/12/29 00:00 [received]
PHST- 2005/01/05 00:00 [accepted]
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2006/11/10 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - 10.1007/s00405-005-0925-2 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2005 Nov;262(11):917-20. doi: 
      10.1007/s00405-005-0925-2. Epub 2005 May 29.