PMID- 15924321
OWN - NLM
STAT- MEDLINE
DCOM- 20051201
LR  - 20131121
IS  - 1522-7235 (Print)
IS  - 1522-7235 (Linking)
VI  - 20
IP  - 3
DP  - 2005 May-Jun
TI  - Determination of MDMA and MDA in rat urine by semi-micro column HPLC-fluorescence 
      detection with DBD-F and their monitoring after MDMA administration to rat.
PG  - 210-5
AB  - A simultaneous semi-micro column HPLC method with fluorescence detection of 
      abused drugs, such as 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in 
      rat urine was examined by using 
      4-(N,N-dimethylaminosulphonyl)-7-fluoro-1,2,3-benzoxadiazole (DBD-F) as a 
      labelling reagent and alpha-phenylethylamine as an internal standard (IS). A 
      sample (50 microL) of rat urine was added to 5 microL IS and 100 microL 100 
      mmol/L borate buffer (pH 12) and extracted with 1.5 mL n-hexane. After 
      evaporation, 50 microL 75 mmol/L borate buffer (pH 8.5) and 50 microL 20 mmol/L 
      DBD-F in CH3CN were added to the residue and mixed well. The resultant solution 
      was heated for 20 min at 80 degrees C and then cooled in an ice bath. A good 
      separation of DBD-derivatives could be achieved within 45 min using a semi-micro 
      ODS column with an eluent of CH3CN/CH3OH/10 mmol/L imidazole-HNO3 buffer (pH 7.0) 
      (= 45:5:50, v/v/v %). The DBD derivatives were monitored at 565 nm with an 
      excitation at 470 nm. The calibration curves showed good linearity (r = 0.997) 
      with 0.5-15 ng/mL detection limits at a S/N ratio of 3. MDMA and MDA in rat urine 
      could be monitored for 15 h after a single administration of MDMA to rat (2.0 
      mg/kg, i.p.). The concentrations for MDMA and MDA (n = 3) were 0.13-160.1 and 
      0.17-10.9 microg/mL, respectively.
FAU - Wada, Mitsuhiro
AU  - Wada M
AD  - Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate 
      School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 
      852-8521, Japan.
FAU - Nakamura, Shinichi
AU  - Nakamura S
FAU - Tomita, Mamoru
AU  - Tomita M
FAU - Nakashima, Mihoko N
AU  - Nakashima MN
FAU - Nakashima, Kenichiro
AU  - Nakashima K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Borates)
RN  - 0 (Diazonium Compounds)
RN  - 0 (p-(N-N-dimethylamino)-benzendiazonium fluoroborate)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*urine
MH  - Animals
MH  - Borates
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Diazonium Compounds
MH  - Drug Monitoring/methods
MH  - Fluorometry
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*urine
MH  - Rats
MH  - Rats, Wistar
MH  - Substance Abuse Detection/*methods/standards
EDAT- 2005/06/01 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - 10.1002/bio.821 [doi]
PST - ppublish
SO  - Luminescence. 2005 May-Jun;20(3):210-5. doi: 10.1002/bio.821.