PMID- 15925211
OWN - NLM
STAT- MEDLINE
DCOM- 20060518
LR  - 20161124
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 38
IP  - 2
DP  - 2005 Jun 15
TI  - Separation of compounds interacting with liposome membrane in combined 
      prescription of traditional Chinese medicines with immobilized liposome 
      chromatography.
PG  - 216-24
AB  - Immobilized liposome chromatography (ILC), the stationary phase of which has been 
      regarded as a mimic biomembranes system was used to separate and analyze 
      compounds interacting with liposome membrane in Danggui Buxue decoction, a 
      combined prescription of traditional Chinese medicines (CPTCMs), and its 
      compositions Radix Astragli and Radix Angelica Sinensis. More than 10 main peaks 
      in the extract of Danggui Buxue decoction were resolved on the ILC column, 
      suggesting that more than 10 components in the prescription have significant 
      retention on ILC column. Ligustilide, astragaloside IV and formononetin, three 
      main bioactive ingredients in Danggui Buxue decoction, were found to have 
      relatively significant, while ferulic acid, another bioactive ingredient in the 
      prescription, relatively weak retention on ILC column. Effects of the eluent pH 
      and amount of immobilized phosphatidylcholine (PC) on separation of interactional 
      compounds in the extract of Danggui Buxue decoction were also investigated. It 
      was found that these two factors strongly affected the retention of some 
      interactional compounds. In addition, the fractions partitioned with different 
      solvents from water extract of this combined prescription were evaluated with 
      this ILC column system.
FAU - Sheng, Liang-Hong
AU  - Sheng LH
AD  - National Chromatographic R&A Center, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116011, China. 
      sh_lianghong@163.com
FAU - Li, Song-Lin
AU  - Li SL
FAU - Kong, Liang
AU  - Kong L
FAU - Chen, Xue-Guo
AU  - Chen XG
FAU - Mao, Xi-Qin
AU  - Mao XQ
FAU - Su, Xing-Ye
AU  - Su XY
FAU - Zou, Han-Fa
AU  - Zou HF
FAU - Li, Ping
AU  - Li P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Coumaric Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Isoflavones)
RN  - 0 (Liposomes)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 0 (danggui buxue decoction)
RN  - 295DQC67BJ (formononetin)
RN  - 3A592W8XKE (astragaloside A)
RN  - AVM951ZWST (ferulic acid)
SB  - IM
MH  - Chromatography/*methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Coumaric Acids/analysis
MH  - Drugs, Chinese Herbal/analysis/chemistry/*isolation & purification
MH  - Hydrogen-Ion Concentration
MH  - Isoflavones/analysis
MH  - Liposomes/*chemistry
MH  - Phosphatidylcholines/chemistry
MH  - Saponins/analysis
MH  - Technology, Pharmaceutical/methods
MH  - Triterpenes/analysis
EDAT- 2005/06/01 09:00
MHDA- 2006/05/19 09:00
CRDT- 2005/06/01 09:00
PHST- 2004/11/26 00:00 [received]
PHST- 2005/01/09 00:00 [revised]
PHST- 2005/01/14 00:00 [accepted]
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2006/05/19 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - S0731-7085(05)00040-3 [pii]
AID - 10.1016/j.jpba.2005.01.008 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2005 Jun 15;38(2):216-24. doi: 10.1016/j.jpba.2005.01.008.