PMID- 15925519
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20061115
IS  - 1046-5928 (Print)
IS  - 1046-5928 (Linking)
VI  - 42
IP  - 2
DP  - 2005 Aug
TI  - Cloning and characterization of the rat HIF-1 alpha prolyl-4-hydroxylase-1 gene.
PG  - 295-304
AB  - Prolyl-4-hydroxylase domain-containing enzymes (PHDs) mediate the 
      oxygen-dependent regulation of the heterodimeric transcription factor 
      hypoxia-inducible factor-1 (HIF-1). Under normoxic conditions, one of the 
      subunits of HIF-1, HIF-1alpha, is hydroxylated on specific proline residues to 
      target HIF-1alpha for degradation by the ubiquitin-proteasome pathway. Under 
      hypoxic conditions, the hydroxylation by the PHDs is attenuated by lack of the 
      oxygen substrate, allowing HIF-1 to accumulate, translocate to the nucleus, and 
      mediate HIF-mediated gene transcription. In several mammalian species including 
      humans, three PHDs have been identified. We report here the cloning of a 
      full-length rat cDNA that is highly homologous to the human and murine PHD-1 
      enzymes and encodes a protein that is 416 amino acids long. Both cDNA and protein 
      are widely expressed in rat tissues and cell types. We demonstrate that purified 
      and crude baculovirus-expressed rat PHD-1 exhibits HIF-1alpha specific prolyl 
      hydroxylase activity with similar substrate affinities and is comparable to human 
      PHD-1 protein.
FAU - Cobb, Ronald R
AU  - Cobb RR
AD  - Systems Biology, Berlex Biosciences, Richmond, CA 94806, USA. 
      Ronald_Cobb@berlex.com
FAU - McClary, John
AU  - McClary J
FAU - Manzana, Warren
AU  - Manzana W
FAU - Finster, Silke
AU  - Finster S
FAU - Larsen, Brent
AU  - Larsen B
FAU - Blasko, Eric
AU  - Blasko E
FAU - Pearson, Jennifer
AU  - Pearson J
FAU - Biancalana, Sara
AU  - Biancalana S
FAU - Kauser, Katalin
AU  - Kauser K
FAU - Bringmann, Peter
AU  - Bringmann P
FAU - Light, David R
AU  - Light DR
FAU - Schirm, Sabine
AU  - Schirm S
LA  - eng
SI  - GENBANK/AY952204
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.11.2 (Procollagen-Proline Dioxygenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Cloning, Molecular
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Procollagen-Proline Dioxygenase/biosynthesis/*chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Spodoptera
EDAT- 2005/06/01 09:00
MHDA- 2006/10/27 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/01/25 00:00 [received]
PHST- 2005/03/21 00:00 [revised]
PHST- 2005/03/25 00:00 [accepted]
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - S1046-5928(05)00112-9 [pii]
AID - 10.1016/j.pep.2005.03.036 [doi]
PST - ppublish
SO  - Protein Expr Purif. 2005 Aug;42(2):295-304. doi: 10.1016/j.pep.2005.03.036.