PMID- 15925987
OWN - NLM
STAT- MEDLINE
DCOM- 20051026
LR  - 20181113
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 72
IP  - 2
DP  - 2005 Apr
TI  - Comparative genetic analysis of metachronous anaplastic oligoastrocytomas with 
      extended recurrence-free interval.
PG  - 95-102
AB  - Two metachronous anaplastic oligoastrocytomas with different cerebral locations 
      were analyzed in a 51-year-old patient with an extended recurrence-free interval 
      of 6 years and an a long survival of 9 years. Remarkably, the patient had not 
      undergone adjuvant chemotherapy. Different cytogenetic and molecular techniques 
      were performed including comparative genomic hybridization (CGH), fluorescence in 
      situ hybridization (FISH), allelic loss analysis, sequencing of p53, 
      p16(INK4a)/CDKN2A and p14(ARF), EGFRamplification studies, investigation of the 
      DNA mismatch repair system as well as tumor clonality. Using CGH and FISH a 
      profile of low accumulation of cytogenetic aberrations was found in the second 
      tumor, with no significant increase in the percentage of hyperdiploid nuclei. 
      Microsatellite analysis showed a common pattern of allelic losses at 1p36, 19q13 
      and 9p21. Both specimens were also similar in that they retained heterozygosity 
      at 10q23-q24 and 13q14 and that they harbor neither EGFR amplification nor 
      mutations of p53, p16(INK4a)/CDKN2A or p14(ARF). The only further alteration in 
      the second tumor was an allelic loss at p53. The X-chromosome inactivation 
      (HUMARA) analysis revealed a polyclonal pattern in both samples. Our data 
      strongly suggest that the second anaplastic oligoastrocytoma developed as a 
      distant relapse of the first tumor. Whether the paucity of accumulation of the 
      observed genetic alterations might be associated with the unusually extended 
      relapse-free time of the patient remains to be elucidated.
FAU - Martinez, Ramon
AU  - Martinez R
AD  - Department of Neurosurgery, University of Dresden, Fetscherstr. 74, D-01307, 
      Dresden, Germany. voelter.martinez@t-online.de
FAU - Schackert, Hans-Konrad
AU  - Schackert HK
FAU - Kirsch, Matthias
AU  - Kirsch M
FAU - Paulus, Werner
AU  - Paulus W
FAU - Joos, Stefan
AU  - Joos S
FAU - Schackert, Gabriele
AU  - Schackert G
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
SB  - IM
MH  - Brain Neoplasms/*genetics/radiotherapy/surgery
MH  - DNA Mutational Analysis
MH  - Disease-Free Survival
MH  - Female
MH  - Gene Amplification
MH  - Genes, erbB-1/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Neoplasms, Second Primary/*genetics/radiotherapy/surgery
MH  - Oligodendroglioma/*genetics/radiotherapy/surgery
MH  - Sequence Analysis, DNA
EDAT- 2005/06/01 09:00
MHDA- 2005/10/27 09:00
CRDT- 2005/06/01 09:00
PHST- 2005/06/01 09:00 [pubmed]
PHST- 2005/10/27 09:00 [medline]
PHST- 2005/06/01 09:00 [entrez]
AID - 10.1007/s11060-004-3347-x [doi]
PST - ppublish
SO  - J Neurooncol. 2005 Apr;72(2):95-102. doi: 10.1007/s11060-004-3347-x.