PMID- 15927426
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20220317
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 353
IP  - 1
DP  - 2005 Jun 20
TI  - Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1.
PG  - 8-15
AB  - We presently describe the full-length cloning and functional characterization of 
      an HIV-1-inducible gene, astrocyte elevated gene (AEG)-1. Additionally, a novel 
      method is outlined for producing tag-free recombinant protein in a baculovirus 
      system and its use in producing AEG-1 protein. AEG-1 mRNA is expressed 
      ubiquitously with higher expression in tissues containing muscular actin and its 
      expression is increased in astrocytes infected with HIV-1 or treated with gp120 
      or tumor necrosis factor (TNF)-alpha. The mRNA encodes a single pass 
      transmembrane protein of predicted molecular mass of 64-kDa and pI 9.3 that 
      predominantly localizes in the endoplasmic reticulum and perinuclear region. 
      Ectopic expression of AEG-1 inhibits excitatory amino acid transporter 2 (EAAT2) 
      promoter activity with the potential to promote glutamate excitotoxicity and 
      consequently HIV-1-associated dementia (HAD). AEG-1 expression is elevated in 
      subsets of breast carcinomas, malignant gliomas and melanomas and it synergizes 
      with oncogenic Ha-ras to enhance soft agar colony forming ability of 
      non-tumorigenic immortalized melanocytes, documenting its tumor promoting 
      activity. AEG-1 may affect tumor progression in multiple cell lineages by 
      augmenting expression of the transformed phenotype and/or by inducing glutamate 
      excitotoxicity in malignant glioma. In these contexts, an HIV-1-inducible gene, 
      AEG-1, may contribute to multiple brain abnormalities, including HAD and tumor 
      formation, by both common and distinct mechanisms.
FAU - Kang, Dong-Chul
AU  - Kang DC
AD  - Hallym University, Ilsong Institute of Life Science, 1605-4, Kwanyang-dong, 
      Anyang, Kyeonggi-do, Republic of Korea.
FAU - Su, Zao-Zhong
AU  - Su ZZ
FAU - Sarkar, Devanand
AU  - Sarkar D
FAU - Emdad, Luni
AU  - Emdad L
FAU - Volsky, David J
AU  - Volsky DJ
FAU - Fisher, Paul B
AU  - Fisher PB
LA  - eng
GR  - P01 NS031492/NS/NINDS NIH HHS/United States
GR  - GM068488/GM/NIGMS NIH HHS/United States
GR  - NS31492/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Antibodies)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA, Complementary)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Glutamate Plasma Membrane Transport Proteins)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (MTDH protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SLC1A2 protein, human)
RN  - 0 (Symporters)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Amino Acid Transport System X-AG/genetics
MH  - Antibodies/immunology
MH  - Astrocytes/drug effects/*metabolism/virology
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Brain
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Excitatory Amino Acid Transporter 2
MH  - Female
MH  - Fetus
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Genetic Vectors/genetics
MH  - Glutamate Plasma Membrane Transport Proteins
MH  - HIV Envelope Protein gp120/pharmacology
MH  - HIV-1/*growth & development
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Male
MH  - Membrane Proteins
MH  - Microscopy, Fluorescence
MH  - Open Reading Frames/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - RNA-Binding Proteins
MH  - Recombinant Proteins/immunology/metabolism
MH  - Symporters/genetics
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism/pharmacology/physiology
EDAT- 2005/06/02 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/06/02 09:00
PHST- 2005/02/07 00:00 [received]
PHST- 2005/03/15 00:00 [revised]
PHST- 2005/04/01 00:00 [accepted]
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - S0378-1119(05)00194-0 [pii]
AID - 10.1016/j.gene.2005.04.006 [doi]
PST - ppublish
SO  - Gene. 2005 Jun 20;353(1):8-15. doi: 10.1016/j.gene.2005.04.006.