PMID- 15927463
OWN - NLM
STAT- MEDLINE
DCOM- 20060406
LR  - 20061115
IS  - 0960-8524 (Print)
IS  - 0960-8524 (Linking)
VI  - 97
IP  - 3
DP  - 2006 Feb
TI  - Effect of temperature and free ammonia on nitrification and nitrite accumulation 
      in landfill leachate and analysis of its nitrifying bacterial community by FISH.
PG  - 459-68
AB  - The cause of seasonal failure of a nitrifying municipal landfill leachate 
      treatment plant utilizing a fixed biofilm was investigated by wastewater analyses 
      and batch respirometric tests at every treatment stage. Nitrification of the 
      leachate treatment plant was severely affected by the seasonal temperature 
      variation. High free ammonia (NH3-N) inhibited not only nitrite oxidizing 
      bacteria (NOB) but also ammonia oxidizing bacteria (AOB). In addition, high pH 
      also increased free ammonia concentration to inhibit nitrifying activity 
      especially when the NH4-N level was high. The effects of temperature and free 
      ammonia of landfill leachate on nitrification and nitrite accumulation were 
      investigated with a semi-pilot scale biofilm airlift reactor. Nitrification rate 
      of landfill leachate increased with temperature when free ammonia in the reactor 
      was below the inhibition level for nitrifiers. Leachate was completely nitrified 
      up to a load of 1.5 kg NH4-N m(-3)d(-1) at 28 degrees C. The activity of NOB was 
      inhibited by NH3-N resulting in accumulation of nitrite. NOB activity decreased 
      more than 50% at 0.7 mg NH3-N L(-1). Fluorescence in situ hybridization (FISH) 
      was carried out to analyze the population of AOB and NOB in the nitrite 
      accumulating nitrifying biofilm. NOB were located close to AOB by forming small 
      clusters. A significant fraction of AOB identified by probe Nso1225 specifically 
      also hybridized with the Nitrosomonas specific probe Nsm156. The main NOB were 
      Nitrobacter and Nitrospira which were present in almost equal amounts in the 
      biofilm as identified by simultaneous hybridization with Nitrobacter specific 
      probe Nit3 and Nitrospira specific probe Ntspa662.
FAU - Kim, Dong-Jin
AU  - Kim DJ
AD  - Department of Environmental System Engineering, Hallym University, 1 Okchon, 
      Chunchon, Kangwon 200-702, Republic of Korea. dongjin@hallym.ac.kr
FAU - Lee, Dong-Ig
AU  - Lee DI
FAU - Keller, Jurg
AU  - Keller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050531
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nitrites)
RN  - 0 (Water Pollutants)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/chemistry/*metabolism
MH  - Biofilms
MH  - Bioreactors/microbiology
MH  - DNA, Bacterial/analysis
MH  - *In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Nitrites/*metabolism
MH  - Nitrobacter/genetics/growth & development
MH  - Nitrosomonas/genetics/growth & development
MH  - Population Dynamics
MH  - *Refuse Disposal
MH  - Soil Microbiology
MH  - *Temperature
MH  - Water Pollutants
EDAT- 2005/06/02 09:00
MHDA- 2006/04/07 09:00
CRDT- 2005/06/02 09:00
PHST- 2003/11/01 00:00 [received]
PHST- 2005/02/21 00:00 [accepted]
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2006/04/07 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - S0960-8524(05)00180-X [pii]
AID - 10.1016/j.biortech.2005.03.032 [doi]
PST - ppublish
SO  - Bioresour Technol. 2006 Feb;97(3):459-68. doi: 10.1016/j.biortech.2005.03.032. 
      Epub 2005 May 31.