PMID- 15927846
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20061115
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 30
IP  - 5
DP  - 2005 Jun 7
TI  - Smad signal and TGFbeta induced apoptosis in human lymphoma cells.
PG  - 228-35
AB  - Transforming growth factor beta1 (TGF beta1) has antiproliferative and/or 
      apoptotic effect on lymphoid cells. In certain lymphomas exogenous TGF beta1 is 
      able to induce apoptosis, however many lymphoid malignancies are resistant to the 
      endogenous TGF beta1 production. We studied the expression and the activity of 
      TGF beta1 signalling components in B cell lymphoma cell lines (e.g. HT 58 cells) 
      and in isolated human peripheral mononuclear cells (PBMCs) from healthy 
      individual's and B-CLL patient's blood. We found that all signal transducer Smads 
      (Smad2,-3; Smad4) and at least one of the inhibitory Smads (Smad6,-7) were 
      expressed in non-treated lymphoma cells, but the inhibitory Smads did not in 
      normal/control PBMCs. However, after TGF beta1 treatment Smad6 disappeared, while 
      the expression of Smad7 increased in HT 58 cells. The activity of Smad signals 
      was proved by phosphorylation of Smad2, nuclear translocation of Smad2/3, and the 
      increased expression of Smad-dependent gene, TIEG in TGF beta1 treated lymphoma 
      cells. These results showed that Smad signaling is available in certain different 
      human lymphoma cells, however ISmads expression could inhibit the signal 
      transmission. This findings indicates that the lost sensitivity of lymphoma cells 
      toward a physiological regulatory factor could be reversed.
FAU - Sebestyen, Anna
AU  - Sebestyen A
AD  - Ist Department of Pathology and Experimental Cancer Research, Faculty of 
      Medicine, Semmelweis University, 1085 Budapest, Hungary. anna@korbl.sote.hu
FAU - Barna, Gabor
AU  - Barna G
FAU - Nagy, Katalin
AU  - Nagy K
FAU - Janosi, Judit
AU  - Janosi J
FAU - Paku, Sandor
AU  - Paku S
FAU - Kohut, Eszter
AU  - Kohut E
FAU - Berczi, Lajos
AU  - Berczi L
FAU - Mihalik, Rudolf
AU  - Mihalik R
FAU - Kopper, Laszlo
AU  - Kopper L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050317
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (SMAD4 protein, human)
RN  - 0 (SMAD6 protein, human)
RN  - 0 (SMAD7 protein, human)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad4 Protein)
RN  - 0 (Smad6 Protein)
RN  - 0 (Smad7 Protein)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/*metabolism
MH  - Humans
MH  - Lymphoma/genetics/*metabolism/*pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - *Signal Transduction
MH  - Smad2 Protein
MH  - Smad3 Protein
MH  - Smad4 Protein
MH  - Smad6 Protein
MH  - Smad7 Protein
MH  - Trans-Activators/*metabolism
MH  - Transforming Growth Factor beta/genetics/*metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2005/06/02 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/06/02 09:00
PHST- 2004/02/23 00:00 [received]
PHST- 2004/10/26 00:00 [revised]
PHST- 2005/01/25 00:00 [accepted]
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - S1043-4666(05)00046-3 [pii]
AID - 10.1016/j.cyto.2005.01.013 [doi]
PST - ppublish
SO  - Cytokine. 2005 Jun 7;30(5):228-35. doi: 10.1016/j.cyto.2005.01.013. Epub 2005 Mar 
      17.