PMID- 15928329
OWN - NLM
STAT- MEDLINE
DCOM- 20050707
LR  - 20061115
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 6
DP  - 2005 Jun
TI  - Microdissection-derived murine mcb probes from somatic cell hybrids.
PG  - 791-2
AB  - The multicolor-banding (mcb) technique is a fluorescence in situ hybridization 
      (FISH)-banding approach, which is based on region-specific microdissection 
      libraries producing changing fluorescence intensity ratios along the chromosomes. 
      The latter are used to assign different pseudocolors to specific chromosomal 
      regions. Here we present the first three available mcb-probe sets for the Mus 
      musculus chromosomes 3, 6, and 18. In the present work, the creation of the 
      microdissection libraries was done for the first time on mouse/human somatic cell 
      hybrids. During creation of the mcb-probes, the latter enabled an unambiguous 
      identification of the, otherwise in GTG-banding, hardly distinguishable murine 
      chromosomes.
FAU - Trifonov, Vladimir
AU  - Trifonov V
AD  - Institut fur Human Genetics and Anthropology, Postfach, D-07740 Jena, Germany.
FAU - Karst, Constanze
AU  - Karst C
FAU - Claussen, Uwe
AU  - Claussen U
FAU - Mrasek, Kristin
AU  - Mrasek K
FAU - Michel, Susanne
AU  - Michel S
FAU - Avner, Philip
AU  - Avner P
FAU - Liehr, Thomas
AU  - Liehr T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromosome Banding/*methods
MH  - Chromosomes, Mammalian/*ultrastructure
MH  - Hybrid Cells/*ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Microdissection
EDAT- 2005/06/02 09:00
MHDA- 2005/07/08 09:00
CRDT- 2005/06/02 09:00
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2005/07/08 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - 53/6/791 [pii]
AID - 10.1369/jhc.4B6598.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Jun;53(6):791-2. doi: 10.1369/jhc.4B6598.2005.