PMID- 15928962
OWN - NLM
STAT- MEDLINE
DCOM- 20060206
LR  - 20220408
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 111
IP  - 4
DP  - 2005 Aug
TI  - Identification and confirmation of three neutral alleles conferring wide 
      compatibility in inter-subspecific hybrids of rice (Oryza sativa L.) using 
      near-isogenic lines.
PG  - 702-10
AB  - Wide-compatibility varieties (WCVs) are a special class of rice germplasm that is 
      able to produce fertile hybrids when crossed to both indica and japonica 
      subspecies. Previous studies determined 'Dular' and 02428 as two WCVs and 
      identified a number of QTLs as having large effects on fertility of 
      inter-subspecific hybrids. In this study, we developed five near-isogenic lines 
      (NILs) for three of the QTLs, f5, f6 and S5, by backcrossing and marker-assisted 
      selection, using "Dular" and 02428 as the donors and "Zhenshan 97" as the 
      recipient. Three of the NILs each carried one introgressed allele, and two NILs 
      each carried two introgressed alleles in combinations. The NILs were testcrossed 
      to an indica tester "Nanjing 11" and a japonica tester "Balilla". The results 
      showed that the f5 allele from "Dular"(f5-Du) is a neutral allele conferring wide 
      compatibility, with a large effect on both pollen and spikelet fertility, and the 
      f6 allele from "Dular" (f6-Du) is a neutral allele for spikelet fertility with 
      smaller effect. The S5 allele from 02428 (S5-08) was confirmed to be a neutral 
      allele for spikelet fertility. It is likely that f6 and S5 are the same locus as 
      deduced by their genomic locations and effects. The results also showed that even 
      in combination, two neutral alleles of different loci were not able to produce 
      normal fertility hybrids in typical indica-japonica crosses. The implications of 
      the findings in rice breeding programs are discussed.
FAU - Wang, G W
AU  - Wang GW
AD  - National Key Laboratory of Crop Genetic Improvement and National Center of Plant 
      Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
FAU - He, Y Q
AU  - He YQ
FAU - Xu, C G
AU  - Xu CG
FAU - Zhang, Qifa
AU  - Zhang Q
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050601
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
SB  - IM
MH  - *Alleles
MH  - Breeding/methods
MH  - Crosses, Genetic
MH  - *Hybridization, Genetic
MH  - Microsatellite Repeats
MH  - Oryza/*genetics
MH  - Pollen/cytology
MH  - *Quantitative Trait Loci
MH  - Reproduction/genetics
EDAT- 2005/06/02 09:00
MHDA- 2006/02/07 09:00
CRDT- 2005/06/02 09:00
PHST- 2005/02/17 00:00 [received]
PHST- 2005/04/22 00:00 [accepted]
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2006/02/07 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - 10.1007/s00122-005-2055-z [doi]
PST - ppublish
SO  - Theor Appl Genet. 2005 Aug;111(4):702-10. doi: 10.1007/s00122-005-2055-z. Epub 
      2005 Jun 1.