PMID- 15929062
OWN - NLM
STAT- MEDLINE
DCOM- 20051103
LR  - 20191210
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 81
IP  - 1
DP  - 2005 Jul 1
TI  - Glial-guided neuronal migration in P19 embryonal carcinoma stem cell aggregates.
PG  - 9-20
AB  - During development of the nervous system, neuronal precursors that originated in 
      proliferative regions migrate along radial glial fibers to reach their final 
      destination. P19 embryonal carcinoma (EC) stem cells exposed to retinoic acid 
      (RA) differentiate into neurons, glia, and fibroblast-like cells. In this work, 
      we induced P19 aggregates for 4 days with RA and plated them onto tissue culture 
      dishes coated with poly-L-lysine. Several cells migrated out of and/or extended 
      processes from the aggregates after 24 hr. Some cell processes were 
      morphologically similar to radial glial fibers and stained for glial fibrillar 
      acidic protein (GFAP) and nestin. Large numbers of migrating cells showed 
      characteristics similar to those of bipolar migrating neurons and expressed the 
      neuronal marker microtubule-associated protein 2. Furthermore, scanning electron 
      microscopy analysis revealed an intimate association between the radial fibers 
      and the migrating cells. Therefore, the migration of neuron-like cells on radial 
      glia fibers in differentiated P19 aggregates resembled some of the migration 
      models used thus far to study gliophilic neuronal migration. In addition, HPTLC 
      analysis in this system showed the expression of 9-O-acetyl GD3, a ganglioside 
      that has been associated with neuronal migration. Antibody perturbation assays 
      showed that immunoblockage of 9-O-acetyl GD3 arrested neuronal migration in a 
      reversible manner. In summary, we have characterized a new cell culture model for 
      investigation of glial-guided neuronal migration and have shown that 9-O-acetyl 
      GD3 ganglioside has an important role in this phenomenon.
CI  - Copyright 2005 Wiley-Liss, Inc.
FAU - Santiago, Marcelo F
AU  - Santiago MF
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, Brazil. felipe@biof.ufrj.br
FAU - Liour, Sean S
AU  - Liour SS
FAU - Mendez-Otero, Rosalia
AU  - Mendez-Otero R
FAU - Yu, Robert K
AU  - Yu RK
LA  - eng
GR  - NS11853/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Gangliosides)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 5688UTC01R (Tretinoin)
RN  - 98743-26-1 (9-O-acetyl-GD3 ganglioside)
SB  - IM
MH  - Animals
MH  - Cell Aggregation/drug effects/physiology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/physiology
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Embryonal Carcinoma Stem Cells
MH  - Gangliosides/*metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Neoplastic Stem Cells/cytology/drug effects/physiology
MH  - Nerve Fibers/*physiology
MH  - Neuroglia/cytology/*physiology
MH  - Neurons/cytology/physiology
MH  - Pluripotent Stem Cells/cytology/*physiology
MH  - Tretinoin/pharmacology
EDAT- 2005/06/02 09:00
MHDA- 2005/11/04 09:00
CRDT- 2005/06/02 09:00
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2005/11/04 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - 10.1002/jnr.20532 [doi]
PST - ppublish
SO  - J Neurosci Res. 2005 Jul 1;81(1):9-20. doi: 10.1002/jnr.20532.