PMID- 15929127
OWN - NLM
STAT- MEDLINE
DCOM- 20051228
LR  - 20191210
IS  - 1545-5009 (Print)
IS  - 1545-5009 (Linking)
VI  - 45
IP  - 6
DP  - 2005 Nov
TI  - Flow cytometry and fluorescence in situ hybridization to detect residual 
      neuroblastoma cells in bone marrow.
PG  - 787-95
AB  - BACKGROUND: In patients with neuroblastoma morphological assessment of BM for 
      residual NB cells is not precise, particularly when the number of tumor cells is 
      small. PROCEDURE: To develop a sensitive and rapid method of detecting NB cells 
      in BM, we assessed the efficiency of flow cytometry (FCM) using markers CD9, 
      CD56, and CD45. The percent of CD9+/CD56+/CD45- (NB phenotype) cells was 
      determined by FCM in 41 samples (16 patients) at various time points. For 
      confirmation fluorescence in situ hybridization (FISH) for 17q gain was 
      performed. RESULTS: Nineteen of the 22 (86%) samples that were negative by 
      morphology were positive by FCM (>0.006% CD9+/CD56+/CD45- cells). The longest 
      time to complete the FCM study was 3 hr. In six FISH experiments the sorted 
      CD9+/CD56+/CD45- population had a higher percentage of cells with 17q gain 
      (11.5-95%) compared to a CD56-/CD45+ internal control population (2-8%). 
      CONCLUSIONS: Our preliminary results suggest that FCM determination of the 
      percent of CD9+/CD56+/CD45- cells is an effective method of rapidly detecting NB 
      cells in BM.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Okcu, Mehmet Fatih
AU  - Okcu MF
AD  - Division of Pediatrics, The University of Texas M.D. Anderson Cancer Center, 
      Houston, TX 77030, USA.
FAU - Wang, Rui-Yu
AU  - Wang RY
FAU - Bueso-Ramos, Carlos
AU  - Bueso-Ramos C
FAU - Schober, Wendy
AU  - Schober W
FAU - Weidner, Douglas
AU  - Weidner D
FAU - Andrassy, Richard
AU  - Andrassy R
FAU - Blakely, Martin
AU  - Blakely M
FAU - Russell, Heidi
AU  - Russell H
FAU - Ozkan, Alp
AU  - Ozkan A
FAU - Kuttesch, John
AU  - Kuttesch J
FAU - Andreeff, Michael
AU  - Andreeff M
FAU - Chan, Ka Wah
AU  - Chan KW
FAU - Ater, Joann
AU  - Ater J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Pediatr Blood Cancer
JT  - Pediatric blood & cancer
JID - 101186624
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Bone Marrow/*pathology
MH  - Bone Marrow Examination
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - Flow Cytometry/methods/standards
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/methods/standards
MH  - Infant
MH  - Male
MH  - Neoplasm, Residual/*diagnosis
MH  - Neuroblastoma/diagnosis/*pathology
MH  - Sensitivity and Specificity
EDAT- 2005/06/02 09:00
MHDA- 2005/12/29 09:00
CRDT- 2005/06/02 09:00
PHST- 2005/06/02 09:00 [pubmed]
PHST- 2005/12/29 09:00 [medline]
PHST- 2005/06/02 09:00 [entrez]
AID - 10.1002/pbc.20428 [doi]
PST - ppublish
SO  - Pediatr Blood Cancer. 2005 Nov;45(6):787-95. doi: 10.1002/pbc.20428.