PMID- 15929826
OWN - NLM
STAT- MEDLINE
DCOM- 20091215
LR  - 20111017
IS  - 0253-3758 (Print)
IS  - 0253-3758 (Linking)
VI  - 33
IP  - 3
DP  - 2005 Mar
TI  - [Upregulating the expression of angiogenesis-related genes by transplanting 
      autologous mononuclear bone marrow cells into myocardial infarction scar and the 
      periphery].
PG  - 260-4
AB  - OBJECTIVE: To detect the regulation of angiogenic genes involved in the processes 
      of collateral development. METHODS: Myocardial infarction (MI) scar was induced 
      by cryoinjury in New Zealand rabbits. Four weeks after MI, 24 hours before cell 
      transplantation, bone marrow was aspirated from the right thigh bone and 
      mononuclear bone marrow cells (BMCs) were isolated by Ficoll density gradient 
      centrifugation. Then the mononuclear BMCs (n = 8) or IMDM culture medium (n = 8) 
      were transplanted into infarction scar and the periphery. Four weeks after 
      mononuclear BMCs transplantation, DNA microarray analysis was performed to detect 
      the regulation of angiogenesis-related genes in infarction scar and the 
      periphery. And the differences of angiogenic genes expression were compared among 
      several important growth factors by Western blot. RESULTS: DNA microarray 
      analysis showed the detail regulation of genes involved in the angiogenic 
      processes. There were 15 genes upregulated over 3 times in the infarction scar. 
      In addition, we also found more genes are involved in the process of angiogenesis 
      in its periphery than in the infarction scar (40 genes vs. 15 genes). Western 
      bolt analysis further demonstrated that mononuclear BMCs transplantation was 
      capable of increasing the levels of VEGF, FGF and Angiopoietin-I expression in 
      the infarction scar and its periphery, compared with the control group, P < 0.05. 
      CONCLUSION: These findings indicate that the natural angiogenic processes leading 
      to collateral development are extremely complex, since many kinds of bone 
      marrow-derived growth factors involved in the processes after mononuclear BMCs 
      transplantation into infarction sites.
FAU - Sun, Yong-xin
AU  - Sun YX
AD  - Department of Cardiovascular Surgery, Shanghai Institute of Cardiovascular 
      Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
FAU - Zhao, Qiang
AU  - Zhao Q
FAU - Wang, Yi-qing
AU  - Wang YQ
FAU - Yang, Cheng
AU  - Yang C
FAU - Pan, Cui-zhen
AU  - Pan CZ
FAU - Han, Pei-pei
AU  - Han PP
FAU - Chen, Rui-zhen
AU  - Chen RZ
FAU - Yang, Ying-zhen
AU  - Yang YZ
FAU - Wang, Ke-qiang
AU  - Wang KQ
FAU - Ge, Jun-bo
AU  - Ge JB
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xin Xue Guan Bing Za Zhi
JT  - Zhonghua xin xue guan bing za zhi
JID - 7910682
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Gene Expression Profiling
MH  - Male
MH  - Monocytes/metabolism
MH  - Myocardial Infarction/genetics/*pathology/*therapy
MH  - Neovascularization, Pathologic/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Rabbits
MH  - Up-Regulation
MH  - Ventricular Remodeling
EDAT- 2005/06/03 09:00
MHDA- 2009/12/16 06:00
CRDT- 2005/06/03 09:00
PHST- 2005/06/03 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2005/06/03 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xin Xue Guan Bing Za Zhi. 2005 Mar;33(3):260-4.