PMID- 15930623
OWN - NLM
STAT- MEDLINE
DCOM- 20050818
LR  - 20081121
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 61
IP  - Pt 6
DP  - 2005 Jun
TI  - Crystallization of soluble urokinase receptor (suPAR) in complex with urokinase 
      amino-terminal fragment (1-143).
PG  - 697-700
AB  - Urokinase-type plasminogen activator (urokinase, uPA) and its receptor, uPAR, 
      have been implicated in cell adhesion, migration, tissue remodelling and 
      tumour-cell invasion. uPAR has three domains and is anchored to membranes by a 
      glycosyl-phosphatidylinositol (GPI) anchor. Recombinant uPAR without its GPI 
      anchor, soluble uPAR (suPAR), tends to oligomerize, making it difficult to 
      crystallize. The amino-terminal fragment (ATF) of uPA is the major 
      receptor-binding determinant in suPAR and binds to suPAR with nanomolar affinity, 
      indistinguishable from membrane-bound uPAR. It is shown that uPA is capable of 
      dissociating the oligomerization of suPAR and the crystallization of the 
      suPAR-ATF complex is reported here. The resulting crystals diffract to 3.1 A 
      using a synchrotron X-ray source.
FAU - Huang, Mingdong
AU  - Huang M
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, People's 
      Republic of China. mhuang@fjirsm.ac.cn
FAU - Mazar, Andrew P
AU  - Mazar AP
FAU - Parry, Graham
AU  - Parry G
FAU - Higazi, Abd Al-Roof
AU  - Higazi AA
FAU - Kuo, Alice
AU  - Kuo A
FAU - Cines, Douglas B
AU  - Cines DB
LA  - eng
GR  - R01-HL60169/HL/NHLBI NIH HHS/United States
GR  - R01-HL67831/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050526
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (PLAUR protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Protein Structure, Tertiary
MH  - Receptors, Cell Surface/*chemistry
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Urokinase-Type Plasminogen Activator/*chemistry
EDAT- 2005/06/03 09:00
MHDA- 2005/08/19 09:00
CRDT- 2005/06/03 09:00
PHST- 2004/07/20 00:00 [received]
PHST- 2005/05/03 00:00 [accepted]
PHST- 2005/06/03 09:00 [pubmed]
PHST- 2005/08/19 09:00 [medline]
PHST- 2005/06/03 09:00 [entrez]
AID - S0907444905014174 [pii]
AID - 10.1107/S0907444905014174 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):697-700. doi: 
      10.1107/S0907444905014174. Epub 2005 May 26.