PMID- 15931072
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 16
IP  - 9
DP  - 2005 Jun 21
TI  - Contribution of glutamate receptors to brain-derived neurotrophic factor-induced 
      elevation of intracellular Ca2+levels.
PG  - 977-80
AB  - In the present study, acute effects of brain-derived neurotrophic factor (BDNF) 
      on intracellular calcium concentration ([Ca2+]i) in cultured visual cortical 
      neurons were investigated. We examined specifically whether pharmacological 
      blockade of glutamatergic receptors interfered with effects of BDNF on [Ca2+]i. 
      We found that blockade of N-methyl-D-aspartate (NMDA) receptors significantly 
      reduced the number of BDNF-responsive neurons, while inhibition of metabotropic 
      glutamate receptors (mGluR) completely prevented the effect of BDNF. By contrast, 
      blockade of alpha-amino-3-hydroxy-5-methyl-4-isoxasole propionic acid (AMPA) 
      receptors did not affect the BDNF-induced increase of [Ca2+]i. Our results thus 
      suggest that glutamate-mediated excitatory pathways are involved in the 
      BDNF-induced rise of [Ca2+]i in visual cortical neurons, and that both mGluR and 
      NMDA receptors play a critical role in this event.
FAU - Yang, Benduan
AU  - Yang B
AD  - Brain Research Center and Department of Ophthalmology, University of British 
      Columbia, Vancouver, British Columbia, Canada.
FAU - Gu, Qiang
AU  - Gu Q
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Sodium Channel Blockers)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - SY7Q814VUP (Calcium)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Calcium/*metabolism
MH  - Diagnostic Imaging/methods
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Embryo, Mammalian
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Female
MH  - Glycine/pharmacology
MH  - In Vitro Techniques
MH  - Neurons/*drug effects/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Receptors, Glutamate/*physiology
MH  - Sodium Channel Blockers/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Visual Cortex/*cytology
EDAT- 2005/06/03 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/06/03 09:00
PHST- 2005/06/03 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/06/03 09:00 [entrez]
AID - 00001756-200506210-00019 [pii]
AID - 10.1097/00001756-200506210-00019 [doi]
PST - ppublish
SO  - Neuroreport. 2005 Jun 21;16(9):977-80. doi: 10.1097/00001756-200506210-00019.