PMID- 15931501
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20220408
IS  - 0032-0935 (Print)
IS  - 0032-0935 (Linking)
VI  - 221
IP  - 5
DP  - 2005 Jul
TI  - Programmed cell death and leaf morphogenesis in Monstera obliqua (Araceae).
PG  - 607-18
AB  - The unusual perforations in the leaf blades of Monstera obliqua (Araceae) arise 
      through programmed cell death early in leaf development. At each perforation 
      site, a discrete subpopulation of cells undergoes programmed cell death 
      simultaneously, while neighboring protoderm and ground meristem cells are 
      unaffected. Nuclei of cells within the perforation site become terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive, 
      indicating that DNA cleavage is an early event. Gel electrophoresis indicates 
      that DNA cleavage is random and does not result in bands that represent multiples 
      of internucleosomal units. Ultrastructural analysis of cells at the same stage 
      reveals misshapen, densely stained nuclei with condensed chromatin, disrupted 
      vacuoles, and condensed cytoplasm. Cell walls within the perforation site remain 
      intact, although a small disk of dying tissue becomes detached from neighboring 
      healthy tissues as the leaf expands and stretches the minute perforation. Exposed 
      ground meristem cells at the rim of the perforation differentiate as epidermal 
      cells. The cell biology of perforation formation in Monstera resembles that in 
      the aquatic plant Aponogeton madagascariensis (Aponogetonaceae; Gunawardena et 
      al. 2004), but the absence of cell wall degradation and the simultaneous 
      execution of programmed cell death throughout the perforation site reflect the 
      convergent evolution of this distinct mode of leaf morphogenesis in these 
      distantly related plants.
FAU - Gunawardena, Arunika H L A N
AU  - Gunawardena AH
AD  - Department of Botany, University of Toronto, Toronto, ON, M5S 1A1, Canada.
FAU - Sault, Kathy
AU  - Sault K
FAU - Donnelly, Petra
AU  - Donnelly P
FAU - Greenwood, John S
AU  - Greenwood JS
FAU - Dengler, Nancy G
AU  - Dengler NG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050602
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
SB  - IM
MH  - Apoptosis/*physiology
MH  - Araceae/growth & development/*physiology/ultrastructure
MH  - Plant Leaves/*growth & development/ultrastructure
EDAT- 2005/06/03 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/06/03 09:00
PHST- 2005/01/12 00:00 [received]
PHST- 2005/03/11 00:00 [accepted]
PHST- 2005/06/03 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/06/03 09:00 [entrez]
AID - 10.1007/s00425-005-1545-1 [doi]
PST - ppublish
SO  - Planta. 2005 Jul;221(5):607-18. doi: 10.1007/s00425-005-1545-1. Epub 2005 Jun 2.