PMID- 15931583
OWN - NLM
STAT- MEDLINE
DCOM- 20050811
LR  - 20161124
IS  - 0032-0943 (Print)
IS  - 0032-0943 (Linking)
VI  - 71
IP  - 5
DP  - 2005 May
TI  - Enhancing effects of Scutellaria baicalensis and some of its constituents on 
      TGF-beta1 gene expression in RAW 264.7 murine macrophage cell line.
PG  - 440-5
AB  - Transforming growth factor beta1 (TGF-beta1) has been implicated as an inhibitor 
      of cell proliferation and a potent inducer of apoptosis. Scutellaria baicalensis 
      Georgi (SbG) has been widely used in Asia and recent investigations have shown 
      that SbG has anticancer, antiviral, and anti-inflammatory effects. The aim of 
      this study is to investigate the modulatory effect of SbG on TGF-beta1 gene 
      expression. By using RAW 264.7 cell line as an in vitro model, the effects of SbG 
      on TGF-beta1 gene expression were evaluated by ELISA, reverse-transcription 
      polymerase chain reaction (RT-PCR) and quantitative PCR. Many inhibitors such as 
      mitogen-activated protein kinase (MAPK) inhibitor (PD98059), p38-MAPK inhibitor 
      (SB203580), NF-kappaB inhibitor (aspirin) and protein kinase C inhibitor (H7) 
      were used to determine the possible signal transduction pathways. The results 
      showed that crude extracts of SbG as well as its pure compounds, baicalin, 
      baicalein and chrysin up-regulated TGF-beta1 gene expression on RAW 264.7 cells 
      in a concentration-dependent manner. However, the flavonoid of SbG, wogonin, did 
      not up-regulate TGF-beta1 expression on gene and protein levels on RAW264.7 
      cells. The facts that aspirin and H7 but not PD98059 and SB203580 blocked the 
      enhancing effect suggested that NF-kappaB and PKC might be involved in 
      baicalin-enhanced TGF-beta1 gene expression. We conclude that SbG up-regulates 
      TGF-beta1 gene expression on RAW264.7 cells through NF-kappaB and PKC pathways 
      and this might provide evidence to explain the therapeutic effect and potential 
      adverse effects on the clinical use of Scutellaria baicalensis Georgi.
FAU - Chuang, Han-Ning
AU  - Chuang HN
AD  - Institute of Traditional Medicine, National Yang-Ming University, Taiwan, ROC.
FAU - Wang, Jir-You
AU  - Wang JY
FAU - Chiu, Jen-Hwey
AU  - Chiu JH
FAU - Tsai, Tung-Hu
AU  - Tsai TH
FAU - Yeh, Sheau-Farn
AU  - Yeh SF
FAU - Fu, Shu-Ling
AU  - Fu SL
FAU - Lui, Wing-Yiu
AU  - Lui WY
FAU - Wu, Chew-Wun
AU  - Wu CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (DNA Primers)
RN  - 0 (Flavanones)
RN  - 0 (Immunologic Factors)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Plant Extracts)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - Animals
MH  - DNA Primers
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flavanones/administration & dosage/pharmacology/therapeutic use
MH  - Gene Expression Regulation, Neoplastic
MH  - Immunologic Factors/administration & dosage/*pharmacology/therapeutic use
MH  - Lymphotoxin-alpha/*biosynthesis
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - *Phytotherapy
MH  - Plant Extracts/administration & dosage/*pharmacology/therapeutic use
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Scutellaria baicalensis
MH  - Signal Transduction
EDAT- 2005/06/03 09:00
MHDA- 2005/08/12 09:00
CRDT- 2005/06/03 09:00
PHST- 2005/06/03 09:00 [pubmed]
PHST- 2005/08/12 09:00 [medline]
PHST- 2005/06/03 09:00 [entrez]
AID - 10.1055/s-2005-864140 [doi]
PST - ppublish
SO  - Planta Med. 2005 May;71(5):440-5. doi: 10.1055/s-2005-864140.