PMID- 15932383
OWN - NLM
STAT- MEDLINE
DCOM- 20051011
LR  - 20220317
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 60
IP  - 7
DP  - 2005 Jul
TI  - Aerobiological and immunochemical studies on Carica papaya L. pollen: an 
      aeroallergen from India.
PG  - 920-6
AB  - BACKGROUND: Carica papaya L. is a fruit yielding tree, wildly grown or cultivated 
      in the tropics and subtropics. Its pollen grain has been reported to be airborne 
      and cause immunoglobulin E (IgE)-mediated hypersensitivity. OBJECTIVE: To conduct 
      long-term aerobiological study on Carica pollen, along with aeroallergenic 
      particles originating from it and to identify vis-a-vis characterize an important 
      IgE-reactive component present in this pollen. METHODS: The seasonal and diurnal 
      periodicities of airborne C. papaya pollen were recorded in a 5-year survey using 
      a Burkard volumetric sampler. The allergenic potential was studied by skin prick 
      tests, IgE-enzyme-linked immunosorbent assay (ELISA) and also by aeroallergen 
      immunoblotting. The total pollen extract was fractionated by Sephacryl S-200 
      column, and out of the eluted five fractions, the maximum IgE-reactive fraction 
      (as found in ELISA inhibition) was resolved into five major subfractions in 
      reverse-phase high-performance liquid chromatography (RP-HPLC). The subfraction 
      with optimum IgE reactivity was studied by activity gel, native and nonreducing 
      sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The 
      homogeneity of the isolated protein fraction was checked by crossed 
      immunoelectrophoresis with rabbit antisera and IgE reactivity was confirmed by 
      ELISA inhibition and immunoblotting using individual patient sera. RESULTS: The 
      Carica pollen occurred in the air round the year with peaks during January and 
      September-October. Among a patient population of 1000, skin-test results showed 
      27.8% +1 level and 5.6% +2/+3 level reactions. In aeroallergen immunoblotting of 
      exposed Burkard tape segments, the detected allergen spots showed a significant 
      correlation with airborne pollen count recorded. The pollen extract elicited loss 
      of IgE reactivity when treated with reducing agent-like beta-mercaptoethanol and 
      heat, but showed six IgE-reactive components in nonreducing IgE-immunoblot. The 
      fraction 1 eluted from Sephacryl S-200 column showed highest IgE reactivity and 
      resolved into five major components in RP-HPLC. Out of these, the fraction 
      showing optimum IgE reactivity in IgE-ELISA inhibition and immunoblotting with 
      patient antisera, elicited esterase activity and found to be a homogenous protein 
      of 100 kDa. CONCLUSION: Carica papaya tree contributes significantly to the 
      aeropollen and aeroallergen load of the suburban outskirts of Calcutta 
      metropolis, India. The pollen extract contains an important IgE-reactive protein 
      component of 100 kDa molecular weight with esterase activity.
FAU - Chakraborty, P
AU  - Chakraborty P
AD  - Department of Botany, Bose Institute, Calcutta, India.
FAU - Ghosh, D
AU  - Ghosh D
FAU - Chowdhury, I
AU  - Chowdhury I
FAU - Roy, I
AU  - Roy I
FAU - Chatterjee, S
AU  - Chatterjee S
FAU - Chanda, S
AU  - Chanda S
FAU - Gupta-Bhattacharya, S
AU  - Gupta-Bhattacharya S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Carica/*immunology
MH  - Female
MH  - Humans
MH  - India
MH  - *Inhalation Exposure
MH  - Male
MH  - Middle Aged
MH  - Pollen/*immunology
MH  - Respiratory Hypersensitivity/immunology
EDAT- 2005/06/04 09:00
MHDA- 2005/10/12 09:00
CRDT- 2005/06/04 09:00
PHST- 2005/06/04 09:00 [pubmed]
PHST- 2005/10/12 09:00 [medline]
PHST- 2005/06/04 09:00 [entrez]
AID - ALL634 [pii]
AID - 10.1111/j.1398-9995.2005.00634.x [doi]
PST - ppublish
SO  - Allergy. 2005 Jul;60(7):920-6. doi: 10.1111/j.1398-9995.2005.00634.x.