PMID- 15933900
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20181113
IS  - 1436-2228 (Print)
IS  - 1436-2228 (Linking)
VI  - 7
IP  - 3
DP  - 2005 May-Jun
TI  - Characterization of eastern oyster (Crassostrea virginica Gmelin) chromosomes by 
      fluorescence in situ hybridization with bacteriophage P1 clones.
PG  - 207-14
AB  - Chromosome identification is an essential step in genomic research, which so far 
      has not been possible in oysters. We tested bacteriophage P1 clones for 
      chromosomal identification in the eastern oyster Crassostrea virginica, using 
      fluorescence in situ hybridization (FISH). P1 clones were labeled with 
      digoxigenin-11-dUTP using nick translation. Hybridization was detected with 
      fluorescein-isothiocyanate-labeled anti-digoxigenin antibodies and amplified with 
      2 layers of antibodies. Nine of the 21 P1 clones tested produced clear and 
      consistent FISH signals when Cot-1 DNA was used as a blocking agent against 
      repetitive sequences. Karyotypic analysis and cohybridization positively assigned 
      the 9 P1 clones to 7 chromosomes. The remaining 3 chromosomes can be separated by 
      size and arm ratio. Five of the 9 P1 clones were sequenced at both ends, 
      providing sequence-tagged sites that can be used to integrate linkage and 
      cytogenetic maps. One sequence is part of the bone morphogenetic protein type 1b 
      receptor, a member of the transforming growth factor superfamily, and mapped to 
      the telomeric region of the long arm of chromosome 2. This study shows that 
      large-insert clones such as P1 are useful as chromosome-specific FISH probes and 
      for gene mapping in oysters.
FAU - Wang, Yongping
AU  - Wang Y
AD  - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, 
      Rutgers University, 6959 Miller Avenue, Port Norris, NJ, 08349, USA.
FAU - Xu, Zhe
AU  - Xu Z
FAU - Pierce, James C
AU  - Pierce JC
FAU - Guo, Ximing
AU  - Guo X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050608
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (Deoxyuracil Nucleotides)
RN  - 0 (digoxigenin-11-deoxyuridine triphosphate)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Bacteriophage P1/genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes/*genetics
MH  - Cloning, Molecular
MH  - Computational Biology
MH  - Deoxyuracil Nucleotides
MH  - Digoxigenin/analogs & derivatives
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Ostreidae/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Tagged Sites
EDAT- 2005/06/04 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/06/04 09:00
PHST- 2004/05/08 00:00 [received]
PHST- 2004/07/17 00:00 [accepted]
PHST- 2005/06/04 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/06/04 09:00 [entrez]
AID - 10.1007/s10126-004-0051-y [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2005 May-Jun;7(3):207-14. doi: 10.1007/s10126-004-0051-y. 
      Epub 2005 Jun 8.