PMID- 15935885
OWN - NLM
STAT- MEDLINE
DCOM- 20051212
LR  - 20081121
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 5
DP  - 2005 May
TI  - Identification of CD8+ T-cell epitopes specific for immediate-early 
      transactivator Rta of Epstein-Barr virus.
PG  - 483-93
AB  - Nasopharyngeal carcinoma (NPC) is a human epithelial tumor with a high incidence 
      in Southern Chinese population, with contributions from Epstein-Barr virus (EBV), 
      human leukocyte antigen (HLA), and environmental factors to its etiology. It has 
      been shown previously that the recognition of immediate-early transactivator Rta 
      of EBV by CD8+ T cells may have a significant impact on controlling EBV and, 
      indirectly, NPC. The current study used two computer-aided prediction methods and 
      competition-based HLA-peptide binding assays to screen for HLA B2704/B4601/B5801 
      restricted T-cell epitopes derived from Rta. HLA tetrameric complexes containing 
      these potential T-cell epitopes were synthesized. Rta-specific CD8+ T-cell 
      responses in healthy virus carriers were then defined by these tetramers and 
      IFN-gamma ELISPOT assays. We clearly demonstrated that healthy virus carriers 
      have detectable Rta-specific CD8+ T cells restricted by B2704 in the circulation. 
      However, there were no B4601/B5801 tetramer-reactive T cells specific for Rta in 
      the peripheral blood of matched/mismatched donors. On the other hand, B4601 
      tetramers containing the computer-predicted B4601 binder EBNA3A (318-326) showed 
      detectable tetramer-reactive T cells in the circulation of healthy virus 
      carriers. topes also elicited IFN-gamma responses as detected by ELISPOT.
FAU - Yu, Hongxiang
AU  - Yu H
AD  - WHO Immunology Centre, Faculty of Medicine, National University of Singapore, 
      Republic of Singapore.
FAU - Srinivasan, Nalini
AU  - Srinivasan N
FAU - Ren, Eechee
AU  - Ren E
FAU - Chan, Sohha
AU  - Chan S
LA  - eng
PT  - Journal Article
DEP - 20050310
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (BRLF1 protein, Human herpesvirus 4)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Trans-Activators)
RN  - 0 (Viral Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - B-Lymphocytes/cytology/immunology
MH  - Binding, Competitive/immunology
MH  - CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Cell Line
MH  - Computer Simulation
MH  - Epitopes, T-Lymphocyte/*immunology/metabolism
MH  - Epstein-Barr Virus Nuclear Antigens/immunology
MH  - HLA-B Antigens/*immunology/metabolism
MH  - Humans
MH  - Immediate-Early Proteins/genetics/*immunology
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Lymphocyte Count
MH  - Peptide Fragments/immunology/metabolism
MH  - Protein Binding/immunology
MH  - Trans-Activators/genetics/*immunology
MH  - Viral Proteins
EDAT- 2005/06/07 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/06/07 09:00
PHST- 2004/10/11 00:00 [received]
PHST- 2005/01/18 00:00 [revised]
PHST- 2005/01/21 00:00 [accepted]
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - S0198-8859(05)00030-3 [pii]
AID - 10.1016/j.humimm.2005.01.017 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 May;66(5):483-93. doi: 10.1016/j.humimm.2005.01.017. Epub 2005 
      Mar 10.