PMID- 15935886
OWN - NLM
STAT- MEDLINE
DCOM- 20051212
LR  - 20061115
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 5
DP  - 2005 May
TI  - HLA incompatibility and immunogenicity of human pancreatic islet preparations 
      cocultured with blood cells of healthy donors.
PG  - 494-500
AB  - Type 1 diabetes mellitus (T1D) is a T-cell-mediated autoimmune disease 
      characterized by the destruction of beta cells in the pancreas. An attractive 
      novel therapy for type 1 diabetes is pancreatic islet transplantation, provided 
      that recurrent islet autoimmunity and allograft rejection can be prevented. We 
      analyzed the response of peripheral blood mononuclear cells (PBMC) from healthy 
      blood donors to human islet-cell preparations with a composition similar to that 
      of islet grafts used in clinical transplantation trials. It was examined whether 
      the degree of major histocompatibility complex incompatibility between PBMC and 
      donor islet cells is related to the degree of proliferative T-cell responses 
      during coculture of human leukocyte antigen (HLA)-matched and mismatched PBMC 
      with human islet cell-preparations (i.e., mixed islet/lymphocyte reaction). 
      Prominent T-cell responses were observed in the vast majority of cases of double 
      HLA class II mismatches. Intermediate T-cell responsiveness was observed in 
      single HLA class II mismatches, whereas HLA matches did not induce a T-cell 
      response. Our results identify the potential immunogenicity of islet preparations 
      transplanted between HLA-DR incompatible subjects regardless of an autoimmune 
      background of the recipient.
FAU - Bouwman, Lee H
AU  - Bouwman LH
AD  - Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands.
FAU - Ling, Zhidong
AU  - Ling Z
FAU - Duinkerken, Gaby
AU  - Duinkerken G
FAU - Pipeleers, Daniel G
AU  - Pipeleers DG
FAU - Roep, Bart O
AU  - Roep BO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050226
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Coculture Techniques
MH  - Female
MH  - HLA-DR Antigens/*immunology
MH  - Haplotypes/immunology
MH  - Humans
MH  - Islets of Langerhans/cytology/*immunology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Middle Aged
MH  - Tissue Donors
EDAT- 2005/06/07 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/06/07 09:00
PHST- 2004/11/30 00:00 [received]
PHST- 2005/01/13 00:00 [revised]
PHST- 2005/01/14 00:00 [accepted]
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - S0198-8859(05)00031-5 [pii]
AID - 10.1016/j.humimm.2005.01.018 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 May;66(5):494-500. doi: 10.1016/j.humimm.2005.01.018. Epub 2005 
      Feb 26.