PMID- 15935891
OWN - NLM
STAT- MEDLINE
DCOM- 20051212
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 5
DP  - 2005 May
TI  - Sequence-based typing of the HLA-A10/A19 group and confirmation of a pseudogene 
      coamplified with A*3401.
PG  - 535-42
AB  - The strategy for sequencing human leukocyte antigen (HLA)-A was based on separate 
      amplification of exons 2 and 3, followed by forward and reverse heterozygous 
      sequencing of the alleles. Validation of the method was obtained by sequencing 11 
      individuals carrying alleles from all different HLA-A allele groups, except *43. 
      All alleles could be correctly identified except A*3401. Unexpected polymorphic 
      positions were identified in exon 3, even in individuals homozygous for A*3401. 
      In addition, the pseudogene HLA-COQ or HLA-DEL linked to A*3401 was coamplified 
      and sequenced. The problem was solved by using different amplification primers 
      for exon 3 with mismatches for the two pseudogenes. A total of 252 unrelated 
      individuals with at least one allele belonging to the A10 or A19 group were typed 
      for HLA-A by this strategy. Ten different alleles were identified in the A10 
      group and 14 in the A19 group. As second allele a further 30 different subtypes 
      from all different groups were sequenced. In 21 individuals, sequencing exon 1 
      was necessary to distinguish A*7401 from A*7402. The sequencing strategy, with 
      separate amplification of the exons, has proven to be a robust method, resulting 
      in reliable and efficient high-resolution HLA-A typing.
FAU - Swelsen, Wendy T N
AU  - Swelsen WT
AD  - Tissue Typing Laboratory, University Hospital Maastricht, Maastricht, The 
      Netherlands.
FAU - Voorter, Christina E M
AU  - Voorter CE
FAU - van den Berg-Loonen, Ella M
AU  - van den Berg-Loonen EM
LA  - eng
PT  - Journal Article
DEP - 20050212
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*34:01 antigen)
RN  - 0 (HLA-A10 antigen)
RN  - 0 (HLA-A19 antigen)
SB  - IM
MH  - Alleles
MH  - Exons/genetics
MH  - Genetic Variation
MH  - HLA-A Antigens/*genetics
MH  - Heterozygote
MH  - Histocompatibility Testing/methods
MH  - Homozygote
MH  - Humans
MH  - Introns/genetics
MH  - Polymerase Chain Reaction
MH  - Pseudogenes/*genetics
MH  - Sequence Analysis, DNA/methods
EDAT- 2005/06/07 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/06/07 09:00
PHST- 2004/09/17 00:00 [received]
PHST- 2004/12/22 00:00 [revised]
PHST- 2005/01/05 00:00 [accepted]
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - S0198-8859(05)00013-3 [pii]
AID - 10.1016/j.humimm.2005.01.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 May;66(5):535-42. doi: 10.1016/j.humimm.2005.01.005. Epub 2005 
      Feb 12.