PMID- 15935987
OWN - NLM
STAT- MEDLINE
DCOM- 20050823
LR  - 20121115
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 439
IP  - 1
DP  - 2005 Jul 1
TI  - Characterization of the C2 subdomain of yeast mitochondrial initiation factor 2.
PG  - 113-20
AB  - The COOH-terminal part of the yeast mitochondrial initiation factor 2 (ymIF2), 
      containing the C2 subdomain, was expressed and purified as a histidine-tagged 
      polypeptide of 137 amino acids. Like the recombinant full-length protein, the C2 
      subdomain binds both formyl-Met-tRNA(f)(Met) and unformylated Met-tRNA(f)(Met) 
      with only a small preference for the former species. Formation of a binary 
      complex between the C2 subdomain or the full-length ymIF2 and initiator tRNA was 
      also assessed by fluorescence measurements. The binding of coumarin-Met-tRNA(f) 
      to either protein caused a blue shift of the coumarin emission spectrum and an 
      increase in anisotropy. Full-length ymIF2 is functionally competent in forming an 
      initiation complex and supporting formation of the first peptide bond on 
      Escherichia coli ribosomes. The results demonstrate that ymIF2 has the same 
      domain structure and biochemical properties of a typical IF2 species as found in 
      bacteria or mammalian mitochondria--but with enhanced ability to bind 
      unformylated initiator Met-tRNA.
FAU - Garofalo, Cristiana
AU  - Garofalo C
AD  - Department of Chemistry and Biochemistry, Institute for Cellular and Molecular 
      Biology, The University of Texas, Austin, TX 78712, USA.
FAU - Kramer, Gisela
AU  - Kramer G
FAU - Appling, Dean R
AU  - Appling DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Coumarins)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (RNA, Transfer, Met)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - A4VZ22K1WT (coumarin)
SB  - IM
MH  - Coumarins/chemistry
MH  - Escherichia coli/chemistry
MH  - Mitochondria/*enzymology
MH  - Peptide Initiation Factors/*chemistry
MH  - Protein Structure, Tertiary
MH  - RNA, Transfer, Met/*chemistry
MH  - Ribosomes/*chemistry
MH  - Saccharomyces cerevisiae/*enzymology
MH  - Saccharomyces cerevisiae Proteins/*chemistry
EDAT- 2005/06/07 09:00
MHDA- 2005/08/24 09:00
CRDT- 2005/06/07 09:00
PHST- 2005/03/24 00:00 [received]
PHST- 2005/04/26 00:00 [revised]
PHST- 2005/05/02 00:00 [accepted]
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/08/24 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - S0003-9861(05)00185-2 [pii]
AID - 10.1016/j.abb.2005.05.003 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2005 Jul 1;439(1):113-20. doi: 10.1016/j.abb.2005.05.003.