PMID- 15937142
OWN - NLM
STAT- MEDLINE
DCOM- 20051006
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 78
IP  - 2
DP  - 2005 Aug
TI  - Receptors and lytic mediators regulating anti-tumor activity by the leukemic 
      killer T cell line TALL-104.
PG  - 359-71
AB  - The major histocompatibility complex nonrestricted cytotoxic leukemic T cell line 
      T acute lymphoblastic leukemia (TALL)-104 is being pursued as a therapeutic agent 
      for cancer. However, the receptors and effector mechanisms responsible for its 
      broad tumoricidal function remain undefined. Here, we examined the roles played 
      by natural cytotoxicity receptors (NCR), killer cell immunoglobulin-like 
      receptors, cytolytic granule components, and tumor necrosis factor (TNF) family 
      members in tumor recognition and lysis by TALL-104 cells. The perforin-granzyme 
      pathway, TNF-related apoptosis-inducing ligand (TRAIL), and Fas were each 
      involved in the lysis of particular tumor targets by TALL-104. Furthermore, 
      phorbol 12-myristate 13-acetate/ionomycin treatment induced surface expression of 
      Fas-L and TRAIL. In addition, supernatants from CD3-stimulated TALL-104 cultures 
      exhibited antiproliferative activity, which was blocked 50-90% by anti-TNF-alpha 
      monoclonal antibody (mAb). Although negative for the NCR natural killer (NK)p44, 
      this cell line was found to express NKp46. An anti-NKp46 antibody strongly 
      blocked TALL-104-mediated lysis of certain targets and directly induced cytokine 
      production, granule release, and redirected lysis responses. Anti-NKG2D and 
      anti-2B4 also stimulated redirected cytotoxicity by TALL-104. By contrast, 
      anti-NKG2A mAb did not stain the cells or inhibit killing responses. 
      Alternatively, KIR3DL2 was detected on TALL-104, and expression of its reported 
      ligand, human leukocyte antigen (HLA)-A, on target cells provided protection from 
      cytotoxicity. Thus, NKp46, NKG2D, and 2B4 are activating receptors, and KIR3DL2 
      is an inhibitory receptor on TALL-104. The data demonstrate the ability of 
      TALL-104 cells to recognize a wide variety of tumors with NK cell receptors and 
      kill them with a broad arsenal of cytolytic effector mechanisms, including 
      cytolytic granules and TNF family ligands.
FAU - Brando, Clara
AU  - Brando C
AD  - The Wistar Institute, Philadelphia, PA, USA.
FAU - Mukhopadhyay, Sunil
AU  - Mukhopadhyay S
FAU - Kovacs, Eniko
AU  - Kovacs E
FAU - Medina, Rosa
AU  - Medina R
FAU - Patel, Pritesh
AU  - Patel P
FAU - Catina, Tracey L
AU  - Catina TL
FAU - Campbell, Kerry S
AU  - Campbell KS
FAU - Santoli, Daniela
AU  - Santoli D
LA  - eng
GR  - 5R01 CA20833/CA/NCI NIH HHS/United States
GR  - CA83859/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050603
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (CD244 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (Carcinogens)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Ionophores)
RN  - 0 (KIR3DL2 protein, human)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRK1 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NCR1 protein, human)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily K)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 1)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
RN  - 0 (Receptors, KIR3DL2)
RN  - 0 (Receptors, Natural Killer Cell)
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFSF10 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Tumor Necrosis Factors)
RN  - 56092-81-0 (Ionomycin)
RN  - EC 3.6.5.2 (GEM protein, human)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adoptive Transfer
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Antigens, CD/immunology
MH  - Apoptosis Regulatory Proteins
MH  - CD3 Complex/immunology
MH  - Carcinogens/pharmacology
MH  - Cell Degranulation/immunology
MH  - Cytotoxicity, Immunologic/drug effects/*immunology
MH  - Fas Ligand Protein
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Immediate-Early Proteins/immunology
MH  - Ionomycin/pharmacology
MH  - Ionophores/pharmacology
MH  - Jurkat Cells
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/drug effects/*immunology
MH  - Membrane Glycoproteins/immunology
MH  - Monomeric GTP-Binding Proteins/immunology
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily K
MH  - Natural Cytotoxicity Triggering Receptor 1
MH  - Neoplasms/immunology/therapy
MH  - Receptors, Immunologic/immunology
MH  - Receptors, KIR
MH  - Receptors, KIR3DL2
MH  - Receptors, Natural Killer Cell
MH  - Signal Transduction/drug effects/*immunology
MH  - Signaling Lymphocytic Activation Molecule Family
MH  - T-Lymphocytes, Cytotoxic/cytology/*immunology
MH  - TNF-Related Apoptosis-Inducing Ligand
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/immunology
MH  - Tumor Necrosis Factors/immunology
MH  - U937 Cells
EDAT- 2005/06/07 09:00
MHDA- 2005/10/07 09:00
CRDT- 2005/06/07 09:00
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/10/07 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - jlb.0604360 [pii]
AID - 10.1189/jlb.0604360 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2005 Aug;78(2):359-71. doi: 10.1189/jlb.0604360. Epub 2005 Jun 3.