PMID- 15939159
OWN - NLM
STAT- MEDLINE
DCOM- 20050809
LR  - 20061115
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 151
IP  - 2-3
DP  - 2005 Jul 16
TI  - Use of the genomic matching technique to complement multiplex STR profiling 
      reduces DNA profiling costs in high volume crimes and intelligence led screens.
PG  - 249-57
AB  - The genomic matching technique (GMT) targets duplicated polymorphic sequences 
      within genomic blocks in the human major histocompatibility complex (MHC), 
      differentiating between individuals at the DNA level using a single primer pair 
      per block. The GMT is currently used to supplement human leukocyte antigen (HLA) 
      typing to match donor and recipient pairs for bone marrow transplantation and has 
      the potential to be employed as a powerful exclusion tool in forensic biology. 
      The GMT is highly reproducible, produces DNA profiles from less than 1 ng of DNA 
      and was successfully employed to profile a range of forensic samples including 
      buccal swabs, handled objects and fingerprints. Furthermore, GMT profiles from a 
      single genomic block in the MHC are likely to be more discriminatory than known 
      highly polymorphic short tandem repeat (STR) loci such as ACTBP2. As such, the 
      GMT can reduce the cost of investigations that require profiling of multiple 
      suspects or samples from one or more crime scenes and could be extended to 
      profile genomic blocks in other polymorphic genetic systems in the human genome.
FAU - Laird, R
AU  - Laird R
AD  - Centre for Forensic Science, School of Anatomy and Human Biology, University of 
      Western Australia, Crawley Ave., Nedlands, WA 6009, Australia.
FAU - Dawkins, R L
AU  - Dawkins RL
FAU - Gaudieri, S
AU  - Gaudieri S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050421
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (DNA Primers)
SB  - IM
MH  - DNA Fingerprinting/economics/*methods
MH  - DNA Primers
MH  - Dermatoglyphics
MH  - *Genome Components
MH  - Haplotypes
MH  - Humans
MH  - Major Histocompatibility Complex/*genetics
MH  - Mouth Mucosa/metabolism
MH  - Polymerase Chain Reaction
MH  - *Tandem Repeat Sequences
EDAT- 2005/06/09 09:00
MHDA- 2005/08/10 09:00
CRDT- 2005/06/09 09:00
PHST- 2005/02/25 00:00 [received]
PHST- 2005/02/25 00:00 [revised]
PHST- 2005/02/26 00:00 [accepted]
PHST- 2005/06/09 09:00 [pubmed]
PHST- 2005/08/10 09:00 [medline]
PHST- 2005/06/09 09:00 [entrez]
AID - S0379-0738(05)00110-6 [pii]
AID - 10.1016/j.forsciint.2005.02.018 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2005 Jul 16;151(2-3):249-57. doi: 
      10.1016/j.forsciint.2005.02.018. Epub 2005 Apr 21.