PMID- 15940052
OWN - NLM
STAT- MEDLINE
DCOM- 20050728
LR  - 20231024
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 79
IP  - 11
DP  - 2005 Jun 15
TI  - Fetal mesenchymal stem-cell engraftment in bone after in utero transplantation in 
      a patient with severe osteogenesis imperfecta.
PG  - 1607-14
AB  - BACKGROUND: Mesenchymal stem cells (MSC) are progenitors of mesenchymal tissues 
      such as bone, cartilage, and adipose. Adult human leukocyte antigen (HLA)-matched 
      MSC have been used in cellular therapies of bone disorders such as osteogenesis 
      imperfecta, with promising results. METHODS: A female fetus with multiple 
      intrauterine fractures, diagnosed as severe osteogenesis imperfecta, underwent 
      transplantation with allogeneic HLA-mismatched male fetal MSC in the 32nd week of 
      gestation. Engraftment analyses of donor cells, immunologic reaction against 
      donor cells, and the well-being of the patient were assessed. RESULTS: At 9 
      months of age, on slides stained for osteocalcin or osteopontin, a centromeric 
      XY-specific probe revealed 0.3% of XY-positive cells in a bone biopsy specimen. 
      Whole Y genome fluorescent in situ hybridization staining showed a median of 7.4% 
      Y-positive cells (range, 6.8%-16.6%). Bone histology showed regularly arranged 
      and configurated bone trabeculae. Patient lymphocyte proliferation against donor 
      MSC was not observed in co-culture experiments performed in vitro after MSC 
      injection. Complementary bisphosphonate treatment was begun at 4 months. During 
      the first 2 years of life, three fractures were noted. At 2 years of corrected 
      age, psychomotor development was normal and growth followed the same channel, -5 
      SD. CONCLUSIONS: The authors' findings show that allogeneic fetal MSC can engraft 
      and differentiate into bone in a human fetus even when the recipient is 
      immunocompetent and HLA-incompatible.
FAU - Le Blanc, Katarina
AU  - Le Blanc K
AD  - Center for Allogeneic Stem Cell Transplantation, Karolinska Institutet, 
      Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. 
      Katarina.leblanc@medhs.ki.se
FAU - Gotherstrom, Cecilia
AU  - Gotherstrom C
FAU - Ringden, Olle
AU  - Ringden O
FAU - Hassan, Moustapha
AU  - Hassan M
FAU - McMahon, Robert
AU  - McMahon R
FAU - Horwitz, Edwin
AU  - Horwitz E
FAU - Anneren, Goran
AU  - Anneren G
FAU - Axelsson, Ove
AU  - Axelsson O
FAU - Nunn, Janice
AU  - Nunn J
FAU - Ewald, Uwe
AU  - Ewald U
FAU - Norden-Lindeberg, Solveig
AU  - Norden-Lindeberg S
FAU - Jansson, Monika
AU  - Jansson M
FAU - Dalton, Ann
AU  - Dalton A
FAU - Astrom, Eva
AU  - Astrom E
FAU - Westgren, Magnus
AU  - Westgren M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Collagen Type I)
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Bone and Bones/pathology
MH  - Collagen/genetics
MH  - Collagen Type I
MH  - DNA Primers
MH  - Female
MH  - *Fetal Tissue Transplantation
MH  - Gestational Age
MH  - HLA-DR Antigens/genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Mesoderm
MH  - Osteogenesis Imperfecta/*therapy
MH  - Pregnancy
MH  - Pregnancy Complications/*therapy
MH  - *Stem Cell Transplantation
MH  - Transplantation Chimera
EDAT- 2005/06/09 09:00
MHDA- 2005/07/29 09:00
CRDT- 2005/06/09 09:00
PHST- 2005/06/09 09:00 [pubmed]
PHST- 2005/07/29 09:00 [medline]
PHST- 2005/06/09 09:00 [entrez]
AID - 00007890-200506150-00021 [pii]
AID - 10.1097/01.tp.0000159029.48678.93 [doi]
PST - ppublish
SO  - Transplantation. 2005 Jun 15;79(11):1607-14. doi: 
      10.1097/01.tp.0000159029.48678.93.