PMID- 15945031
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20131121
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 19
IP  - 13
DP  - 2005
TI  - Analysis of organochlorines in harbor seal (Phoca vitulina) tissue samples from 
      Alaska using gas chromatography/ion trap mass spectrometry by an isotopic 
      dilution technique.
PG  - 1815-21
AB  - A gas chromatography/ion trap mass spectrometry (GC/ITMS) method was developed 
      for the determination of organochlorine pesticides (OCPs), polychlorinated 
      biphenyls (PCBs) and polychlorinated naphthalenes (PCNs) in harbor seal (Phoca 
      vitulina) tissues. Tissue samples were homogenized, lyophilized and fortified 
      with (13)C-PCBs 28, 123, 169 and 170, and then extracted with an accelerated 
      solvent extractor with a mixture of hexane and methylene chloride (1:1, v/v). 
      After lipid removal using a 40% H(2)SO(4)-modified silica gel column, all 
      organochlorines were collected in one fraction and further fractionated with an 
      activated carbon/silica gel (1:20) column into a first fraction containing OCPs, 
      non-coplanar PCBs and (13)C-PCBs 28, 123 and 170, and a second containing PCNs, 
      coplanar PCBs and (13)C-PCB 169. Prior to GC/MS/MS analysis, (13)C-PCB 169 was 
      added into the first fraction as an injection standard and (13)C-PCB 170 into the 
      second fraction to calibrate the recoveries of the fortified internal standards. 
      This method can effectively eliminate matrix interferences, and has high 
      selectivity and sensitivity. Recoveries averaged 45-86% for OCPs with relative 
      standard deviations (RSDs) of 2-14%, 52-137% for PCBs with RSDs of 3-29% and 
      36-152% for PCNs with RSDs of 7-29% from lard and chicken heart samples, which 
      were used as alternative matrices to harbor seal samples in recovery studies. The 
      limits of detection for OCPs, PCBs and PCNs were 0.7-1.9, 1.5-8.9 and 0.5-10 pg/g 
      dry weight, respectively. This method can be used to analyze OCPs, PCBs and PCNs 
      in harbor seal blubber, liver and kidney samples.
CI  - Copyright (c) 2005 John Wiley & Sons, Ltd.
FAU - Wang, Dongli
AU  - Wang D
AD  - Department of Molecular Biosciences and Bioengineering, University of Hawaii, 
      Honolulu, Hawaii 96822, USA.
FAU - Atkinson, Shannon
AU  - Atkinson S
FAU - Hoover-Miller, Anne
AU  - Hoover-Miller A
FAU - Li, Qing X
AU  - Li QX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Insecticides)
RN  - 0 (Pesticide Residues)
RN  - 0 (Solvents)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Alaska
MH  - Animals
MH  - Chickens
MH  - Environmental Pollutants/*analysis
MH  - Hydrocarbons, Chlorinated/*analysis
MH  - Indicators and Reagents
MH  - Insecticides/analysis
MH  - Mass Spectrometry
MH  - Myocardium/chemistry
MH  - Pesticide Residues
MH  - Phoca/*metabolism
MH  - Polychlorinated Biphenyls/analysis
MH  - Quality Control
MH  - Radioisotope Dilution Technique
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Solvents
MH  - Specimen Handling
EDAT- 2005/06/10 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/06/10 09:00
PHST- 2005/06/10 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/06/10 09:00 [entrez]
AID - 10.1002/rcm.1990 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2005;19(13):1815-21. doi: 10.1002/rcm.1990.