PMID- 15946241
OWN - NLM
STAT- MEDLINE
DCOM- 20050914
LR  - 20061115
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 14
IP  - 7
DP  - 2005 Jul
TI  - Pigmentation in basal cell carcinoma involves enhanced endothelin-1 expression.
PG  - 528-34
AB  - Basal cell carcinoma (BCC) is the most prevalent malignant skin tumor. In Asian 
      patients, marked pigmentation in BCC lesions is often observed. Recently, 
      endothelins (ETs) have been implicated to participate in the pigmentation process 
      of BCC. Therefore, we set out to investigate the involvement of ET in the 
      pigmentation process of BCC and the potential regulators in the pigmentation 
      pathway. We explored the effects of an established BCC cell line on melanocytes. 
      The growth factor profiles of BCC culture supernatant and effects of supernatant 
      on melanocytes were documented. Potential regulators involved in the pigmentation 
      pathway were also studied. The immunohistochemical staining of pigmented and 
      non-pigmented BCC specimens was performed to confirm our in vitro findings. Our 
      results showed that BCC supernatant contained significant amount of ET-1, basic 
      fibroblast growth factor, and nerve growth factor. Furthermore, BCC supernatant 
      stimulated melanin formation of cultured melanocytes. Addition of ET-receptor 
      antagonist abrogated the melanogenic effect of BCC supernatant on melanocytes. 
      Introduction of UVB irradiation decreased the ET-1 secretion by BCC cells. 
      Immunohistochemical staining of the pigmented facial BCC specimens showed 
      prominent expression of ET-1 on pigmented BCC, while the non-pigmented facial BCC 
      specimens showed little ET-1 reactivity. Tumor necrosis factor-alpha (TNF-alpha) 
      staining showed little expression on BCC specimens, regardless of pigmentation 
      status. In summary, our results indicate that enhanced ET-1 expression in 
      pigmented BCC plays an important role in the hyperpigmentation of this tumor. 
      Moreover, this enhanced ET-1 cascade showed little correlation with UV 
      irradiation and TNF-alpha expression in our study.
FAU - Lan, Cheng-Che E
AU  - Lan CC
AD  - Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung, 
      Taiwan.
FAU - Wu, Ching-Shuang
AU  - Wu CS
FAU - Cheng, Chiu-Min
AU  - Cheng CM
FAU - Yu, Chia-Li
AU  - Yu CL
FAU - Chen, Gwo-Shing
AU  - Chen GS
FAU - Yu, Hsin-Su
AU  - Yu HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Endothelin-1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Carcinoma, Basal Cell/*metabolism/pathology
MH  - Cell Movement
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Endothelin-1/*metabolism
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratinocytes/cytology/metabolism
MH  - Melanocytes/cytology/metabolism
MH  - Nerve Growth Factor/metabolism
MH  - Pigmentation/*physiology
MH  - Skin Neoplasms/*metabolism/pathology
EDAT- 2005/06/11 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
AID - EXD320 [pii]
AID - 10.1111/j.0906-6705.2005.00320.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2005 Jul;14(7):528-34. doi: 10.1111/j.0906-6705.2005.00320.x.