PMID- 15946258
OWN - NLM
STAT- MEDLINE
DCOM- 20050729
LR  - 20240413
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 115
IP  - 3
DP  - 2005 Jul
TI  - Inhibition of immunoglobulin E synthesis through Fc gammaRII (CD32) by a 
      mechanism independent of B-cell receptor co-cross-linking.
PG  - 407-15
AB  - The inhibitory effect on antibody production by immune complexes has been shown 
      to depend on co-ligation of the B-cell antigen receptor (BCR) with the 
      low-affinity receptor for immunoglobulin G (IgG) (Fc gammaRIIb, CD32). Here we 
      report that immunoglobulin E (IgE) synthesis, induced in a BCR-independent manner 
      by interleukin-4 (IL-4) and anti-CD40 antibody, was inhibited by CD32 ligation. 
      The observed effect was specific for CD32 as, first, antibodies directed against 
      other B-cell surface structures had no inhibitory effect, and, second, treatment 
      with anti-CD32 of cells that had been in culture for 2 days was ineffective owing 
      to the down-regulation of CD32 expression. IgE inhibition was also observed in 
      cells stimulated by IL-4/CD40 F(ab')(2) or IL-4 plus soluble CD40 ligand, 
      demonstrating that co-cross-linking of CD32 and CD40 was not necessary to induce 
      inhibition. Mechanistic studies into the IgE class switch process demonstrated 
      that IL-4/anti-CD40-induced IgE germline gene transcription and B-cell 
      proliferation were not affected by CD32 ligation. The data demonstrate that the 
      negative regulatory role of the CD32 molecule is not restricted to BCR-induced 
      B-cell activation, but is also functional on other B-cell activation pathways 
      mediated by CD40 and IL-4.
FAU - Horejs-Hoeck, Jutta
AU  - Horejs-Hoeck J
AD  - University of Salzburg, Salzburg, Austria.
FAU - Hren, Andrea
AU  - Hren A
FAU - Mudde, Geert C
AU  - Mudde GC
FAU - Woisetschlager, Maximilian
AU  - Woisetschlager M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (CD40 Antigens)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, IgG)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibody Specificity/immunology
MH  - B-Lymphocytes/*immunology
MH  - CD40 Antigens/immunology
MH  - Cell Division/immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Down-Regulation/immunology
MH  - Humans
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis/immunology
MH  - Immunoglobulin Isotypes/immunology
MH  - Immunoglobulin M/biosynthesis
MH  - Interleukin-4/immunology
MH  - Ligands
MH  - Palatine Tonsil/immunology
MH  - Receptors, Antigen, B-Cell/*immunology
MH  - Receptors, IgG/*immunology
MH  - Transcription, Genetic/immunology
PMC - PMC1782155
EDAT- 2005/06/11 09:00
MHDA- 2005/07/30 09:00
PMCR- 2006/07/01
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2005/07/30 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
PHST- 2006/07/01 00:00 [pmc-release]
AID - IMM2162 [pii]
AID - 10.1111/j.1365-2567.2005.02162.x [doi]
PST - ppublish
SO  - Immunology. 2005 Jul;115(3):407-15. doi: 10.1111/j.1365-2567.2005.02162.x.