PMID- 15946474
OWN - NLM
STAT- MEDLINE
DCOM- 20070313
LR  - 20150313
VI  - 24
IP  - 6
DP  - 2005 Jun
TI  - [In vitro induction of mature dendritic cells from malignant pleural effusions].
PG  - 663-6
AB  - BACKGROUND & OBJECTIVE: Dendritic cells (DCs) can activate immunologic naive T 
      cells to initiate antigen-specific immune responses. This study was to in vitro 
      induce mature DCs from malignant pleural effusions of patients with lung cancer. 
      METHODS: Malignant pleural effusions (500-1 000 ml) were collected from 16 
      patients with primary lung cancer. Precursory DCs were obtained through density 
      gradient centrifugation and magnetic cell sorting from malignant pleural 
      effusions, and cultured with interleukin-4 (IL-4), granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-alpha). 
      Morphology of DCs was observed under invert optical microscope and electronic 
      microscope; phenotype of DCs was analyzed by flow cytometry. Effect of DCs on 
      proliferation of tumor infiltrative lymphocytes (TILs) was observed through mixed 
      lymphocyte reaction (MLR). RESULTS: Mature DCs with typical morphology (elongated 
      dendritic processions observed under invert optical microscope and electronic 
      microscope) were induced from malignant pleural effusions. Expressions of surface 
      phenotypes were higher in DCs induced for 48 h than in DCs induced for 0, 24, 96, 
      and 192 h. When activated with DCs, proliferation of TILs was enhanced. 
      CONCLUSION: Mature DCs could be induced from malignant pleural effusions of 
      patients with lung cancer.
FAU - Huang, Hui
AU  - Huang H
AD  - Department of Clinical Tumor, The Second Affiliated Hospital, Guangzhou Medical 
      College, Guangzhou, Guangdong 510260, P. R. China. gzhhui123@163.com
FAU - Zeng, Bo-Hang
AU  - Zeng BH
FAU - Chen, Jing-Qi
AU  - Chen JQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adenocarcinoma/pathology
MH  - Aged
MH  - Carcinoma, Small Cell/pathology
MH  - Carcinoma, Squamous Cell/pathology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*physiology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Lung Neoplasms/*pathology
MH  - Lymphocytes, Tumor-Infiltrating/*cytology
MH  - Male
MH  - Middle Aged
MH  - Pleural Effusion, Malignant/*pathology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2005/06/11 09:00
MHDA- 2007/03/14 09:00
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
AID - 1000467X2005060663 [pii]
PST - ppublish
SO  - Ai Zheng. 2005 Jun;24(6):663-6.