PMID- 15946509
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20171116
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 26
IP  - 1
DP  - 2005 Jan
TI  - [Clonal study on single apoptotic cells in myelodysplastic syndromes].
PG  - 39-43
AB  - OBJECTIVE: To investigate the clonal origin of the apoptotic hematopoietic cells 
      in myelodysplastic syndromes (MDS). METHODS: Nineteen patients with MDS whose 
      karyotypes had been analysed with G- or R-binding were studied. ISEL (DNA in situ 
      end labelling) and FISH (fluorescence in situ hybridization) (simplified as 
      ISEL/FISH) were performed simultaneously in a single nucleated cells on cytospin 
      slides in 10/19 of the patients. The ratio of FISH positive cells to all 
      nucleated cells and to apoptotic cells were counted. Eight slides from 4 normal 
      donors induced apoptosis by Fas monoclonal antibody (McAb) were used as 
      methodology control. For the rest 9 patients, different fractions of cells (PI-, 
      Annexin V+PI-, Annexin V-PI-) were sorted by flow cytometry and then cytospined 
      for FISH analysis (simplified as FCM/FISH). RESULTS: The mean percentage of 
      abnormal clonal cells in nuclear cells in the 10 cases detected by ISEL/FISH was 
      37.1%, whereas it was 24.0% in apoptotic cells. Induction by Fas McAb didn't lead 
      to change of karyotype. Moreover, 8/9 patients analysed by FCM/FISH showed higher 
      cell proportion with normal karyotypes in apoptosis-undergoing cells than that in 
      non-apoptotic cells. CONCLUSION: Over apoptotic hematopoietic cells in MDS are 
      mainly residual normal hematopoietic cells.
FAU - Li, Xiao
AU  - Li X
AD  - Department of Hematology, the Sixth People's Hospital Affiliated to Jiaotong 
      University, Shanghai 200233, China.
FAU - Shen, Wei-ming
AU  - Shen WM
FAU - Pu, Quan
AU  - Pu Q
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (fas Receptor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Apoptosis
MH  - Bone Marrow Cells/metabolism/*pathology
MH  - Cells, Cultured
MH  - Clone Cells/metabolism/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Situ Nick-End Labeling
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/genetics/metabolism/*pathology
MH  - fas Receptor/metabolism
EDAT- 2005/06/11 09:00
MHDA- 2009/01/27 09:00
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2005 Jan;26(1):39-43.