PMID- 15946559
OWN - NLM
STAT- MEDLINE
DCOM- 20060509
LR  - 20211203
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 27
IP  - 3
DP  - 2005 Mar
TI  - [Mutation of p53 and overexpression of STK15 in laryngeal squamous-cell 
      carcinoma].
PG  - 134-7
AB  - OBJECTIVE: To explore the relationship between p53 gene mutations and STK15 
      abnormal expression in the development of human laryngeal squamous-cell carcinoma 
      (LSCC). METHODS: LSCC tissues and matched normal tissues were taken during 
      operation from 55 patients without previous chemotherapy or radiotherapy. 
      Following polymerase chain reaction amplification direct sequencing single strand 
      conformational polymorphism (PCR-SSCP) combined with silver staining were used to 
      detect mutations of p53 gene in exons 7 and 8 (p53E7 and p53E8) using genomic DNA 
      from 110 specimens including 55 LSCC tissues and 55 matched normal tissues. STK15 
      expression were evaluated by RT-PCR with beta-actin as internal control. RESULTS: 
      The mutation rate of p53E7 was 30.9% (compared to normal tissues, chi(2) = 8.66, 
      P < 0.01). There was no mutation in p53E8. In 38 of the 55 cases (69.1%), the 
      STK15 mRNA expression level was higher than that of the paired normal tissue. The 
      STK15 to beta-actin ratio of average density value was 1.22 +/- 0.49 in the 
      cancer tissue, and 0.99 +/- 0.54 in the normal tissues (t = 4.539, P < 0.01). In 
      14 of the 17 cases (82.4%) with p53E7 mutations, the STK15 expression was higher 
      than that of normal tissue. In the 38 cases with STK15 over-expression, p53E7 
      mutation was found in 14 cases (36.8%). The rate of concurrence of p53E gene 
      mutations and STK15 over-expression (25.5%) was higher than that of only p53E 
      gene mutations (chi(2) = 26.025, P < 0.01). CONCLUSION: There is significant 
      association between p53 gene mutation and STK15 over-expression in laryngeal 
      squamous-cell carcinoma.
FAU - Zhao, Xu
AU  - Zhao X
AD  - Department of Medical Genetics, China Medical University, Shenyang 110001, China.
FAU - Li, Fu-cai
AU  - Li FC
FAU - Li, Ying-hui
AU  - Li YH
FAU - Fu, Wei-neng
AU  - Fu WN
FAU - Huang, Dai-fa
AU  - Huang DF
FAU - Ye, Yan
AU  - Ye Y
FAU - Xu, Zhen-ming
AU  - Xu ZM
FAU - Sun, Kai-lai
AU  - Sun KL
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (Actins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (AURKA protein, human)
RN  - EC 2.7.11.1 (Aurora Kinase A)
RN  - EC 2.7.11.1 (Aurora Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Aurora Kinase A
MH  - Aurora Kinases
MH  - Carcinoma, Squamous Cell/*genetics/metabolism
MH  - Exons
MH  - *Frameshift Mutation
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism
MH  - Mutation, Missense
MH  - Protein Serine-Threonine Kinases/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2005/06/11 09:00
MHDA- 2006/05/10 09:00
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2006/05/10 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2005 Mar;27(3):134-7.