PMID- 15948240
OWN - NLM
STAT- MEDLINE
DCOM- 20050831
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 22
DP  - 2005 Jun 14
TI  - Expression and immunoreactivity of an epitope of HCV in a foreign epitope 
      presenting system.
PG  - 3363-7
AB  - AIM: To construct and highly express an epitope of hepatitis C virus (HCV) in a 
      foreign epitope presenting vector based on an insect virus, and to study the 
      antigenicity of the epitope. METHODS: The HCV epitope sequence (amino acid 
      residues 315 to 328: EGHRMAWDMMMNWS) of the E1 region was constructed at 
      different positions of a foreign epitope presenting vector based on an insect 
      virus, flock house virus (FHV) capsid protein encoding gene as a vector, and 
      expressed in E. coli cells. Western blotting and ELISA were used to detect the 
      immunoreactivity of these recombinant proteins. RESULTS: The gene encoding of the 
      concerned B-cell epitope of HCV E1 envelope protein was expressed on FHV capsid 
      carrier protein at positions I1 (aa 106), I2 (aa 153) and I3 (aa 305), 
      respectively, on the surface of FHV capsid protein. The recombinant proteins in 
      this system could be highly expressed in more than 40% of total cell protein of 
      E. coli BL21. All the expressed recombinant proteins were in inclusion body form, 
      and showed obvious immunoreactivity by Western blotting. Further purified 
      recombinant proteins were detected by indirect ELISA as coating antigen 
      respectively. All recombinant proteins could still show immunoreactivity. 
      CONCLUSION: The epitope of HCV E1 envelope protein can be highly expressed in FHV 
      carrier system as a chimeric protein with high immunoreactivity. This system has 
      multiple entry sites conferring many possible conformations closer to the native 
      one for a given sequence.
FAU - Peng, Mei
AU  - Peng M
AD  - Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of 
      Medical Sciences/Peking Union Medical College, 379 Jiaoling Road, Kunming 650118, 
      Yunnang Province, China. mpjpm@public.km.yn.cn
FAU - Dai, Chang-Bai
AU  - Dai CB
FAU - Chen, Yuan-Ding
AU  - Chen YD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Capsid Proteins)
RN  - 0 (E1 protein, Hepatitis C virus)
RN  - 0 (Epitopes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*genetics/*immunology
MH  - Capsid Proteins/genetics/immunology
MH  - Epitopes/*genetics/*immunology
MH  - Escherichia coli
MH  - Hepacivirus/*genetics/*immunology
MH  - Humans
MH  - Insecta
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Viral Envelope Proteins/genetics/immunology
PMC - PMC4315989
EDAT- 2005/06/11 09:00
MHDA- 2005/09/01 09:00
PMCR- 2005/06/14
CRDT- 2005/06/11 09:00
PHST- 2005/06/11 09:00 [pubmed]
PHST- 2005/09/01 09:00 [medline]
PHST- 2005/06/11 09:00 [entrez]
PHST- 2005/06/14 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i22.3363 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Jun 14;11(22):3363-7. doi: 10.3748/wjg.v11.i22.3363.