PMID- 15949143
OWN - NLM
STAT- MEDLINE
DCOM- 20050823
LR  - 20240109
IS  - 0968-0519 (Print)
IS  - 0968-0519 (Linking)
VI  - 11
IP  - 3
DP  - 2005
TI  - Differential maturation of murine bone-marrow derived dendritic cells with 
      lipopolysaccharide and tumor necrosis factor-alpha.
PG  - 145-60
AB  - Dendritic cells (DCs) play a key role in the interface between the innate and 
      acquired immune systems. In response to both exogenous as well as endogenous 
      signals, DCs undergo a programmed maturation to become an efficient, 
      antigen-presenting cell. Yet little is known regarding the differential responses 
      by endogenous versus exogenous stimuli on DC maturation. In the present report, 
      we have compared the phenotypic, functional, and genome-wide expression responses 
      associated with maturation by bone marrow derived DCs to either an endogenous 
      danger signal, tumor necrosis factor-alpha (TNF-alpha), or a microbial product, 
      bacterial lipopolysaccharide (LPS). Examination of the cell surface expression of 
      DCs as well as cytokine production demonstrated that patterns of DC maturation 
      varied dramatically depending upon the stimulus. Whereas LPS was highly effective 
      in terms of inducing phenotypic and functional maturation, TNF-alpha exposure 
      produced a phenotypically distinct DC. Gene expression patterns in DCs 6 and 24 h 
      after LPS and TNF-alpha exposure revealed that these activation signals produce 
      fundamentally different genomic responses. Supervised analysis revealed that the 
      expression of 929 probe sets discriminated among the treatment groups, and the 
      patterns of gene expression in TNF-alpha stimulated DCs were more similar to 
      unstimulated cells at both 6 and 24 h post-stimulation than to LPS-stimulated 
      cells at the same time points. These findings reveal that DCs are capable of a 
      varying phenotypic response to different antigens and endogenous signals.
FAU - Efron, Philip A
AU  - Efron PA
AD  - Department of Surgery, University of Florida College of Medicine, Gainesville, 
      32610, USA.
FAU - Tsujimoto, Hironori
AU  - Tsujimoto H
FAU - Bahjat, Frances R
AU  - Bahjat FR
FAU - Ungaro, Ricardo
AU  - Ungaro R
FAU - Debernardis, Justin
AU  - Debernardis J
FAU - Tannahill, Cynthia
AU  - Tannahill C
FAU - Baker, Henry V
AU  - Baker HV
FAU - Edwards, Carl K
AU  - Edwards CK
FAU - Moldawer, Lyle L
AU  - Moldawer LL
LA  - eng
GR  - R01 GM63041-03/GM/NIGMS NIH HHS/United States
GR  - R37 GM-40561-15/GM/NIGMS NIH HHS/United States
GR  - T32 GM-08721-04/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Endotoxin Res
JT  - Journal of endotoxin research
JID - 9433350
RN  - 0 (Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens
MH  - Bone Marrow Cells
MH  - *Cell Differentiation
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Inflammation
MH  - Lipopolysaccharides/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2005/06/14 09:00
MHDA- 2005/08/24 09:00
CRDT- 2005/06/14 09:00
PHST- 2005/06/14 09:00 [pubmed]
PHST- 2005/08/24 09:00 [medline]
PHST- 2005/06/14 09:00 [entrez]
AID - 10.1179/096805105X46583 [doi]
PST - ppublish
SO  - J Endotoxin Res. 2005;11(3):145-60. doi: 10.1179/096805105X46583.