PMID- 15949805
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20061115
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 579
IP  - 16
DP  - 2005 Jun 20
TI  - Grifolin, a potential antitumor natural product from the mushroom Albatrellus 
      confluens, inhibits tumor cell growth by inducing apoptosis in vitro.
PG  - 3437-43
AB  - Grifolin is a natural biologically active substance isolated from the fresh 
      fruiting bodies of the mushroom Albatrellus confluens. Here, for the first time, 
      we describe a novel activity of grifolin, namely its ability to inhibit the 
      growth of tumor cells by the induction of apoptosis. Grifolin strongly inhibited 
      the growth of tumor cell lines: CNE1, HeLa, MCF7, SW480, K562, Raji and B95-8. 
      Analysis of acridine orange (AO)/ethidium bromide (EB) staining and flow 
      cytometry showed that grifolin possessed apoptosis induction activity to CNE1, 
      HeLa, MCF7 and SW480. Furthermore, the cytochrome c release from mitochondria was 
      detected by confocal microscopy in CNE1 cells after a 12h treatment with 
      grifolin. The increase of caspase-8, 9, 3 activities revealed that caspase was a 
      key mediator of the apoptotic pathway induced by grifolin, and the 
      underexpression of Bcl-2 and up-regulation of Bax resulted in the increase of 
      Bax: Bcl-2 ratio, suggesting that Bcl-2 family involved in the control of 
      apoptosis. Owing to the combination of the significant antitumor activity by 
      inducing apoptosis and natural abundance of the compound, grifolin holds the 
      promise of being an interesting antitumor agent that deserves further laboratory 
      and in vivo exploration.
FAU - Ye, Mao
AU  - Ye M
AD  - Cancer Research Institute, Xiangya School of Medicine, Central South University, 
      Changsha, Hunan 410078, PR China.
FAU - Liu, Ji-Kai
AU  - Liu JK
FAU - Lu, Zhong-Xin
AU  - Lu ZX
FAU - Zhao, Yan
AU  - Zhao Y
FAU - Liu, Su-Fang
AU  - Liu SF
FAU - Li, Li-Li
AU  - Li LL
FAU - Tan, Ming
AU  - Tan M
FAU - Weng, Xin-Xian
AU  - Weng XX
FAU - Li, Wei
AU  - Li W
FAU - Cao, Ya
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antineoplastic Agents)
RN  - 0 (BAX protein, human)
RN  - 0 (Bax protein, mouse)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Terpenes)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 6379-55-1 (grifolin)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - *Apoptosis
MH  - Basidiomycota/chemistry
MH  - Caspases/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cytochromes c/metabolism
MH  - Down-Regulation
MH  - Humans
MH  - Mice
MH  - Neoplasms/pathology
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Terpenes/*pharmacology
MH  - Up-Regulation
MH  - bcl-2-Associated X Protein
EDAT- 2005/06/14 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/06/14 09:00
PHST- 2005/04/25 00:00 [received]
PHST- 2005/05/03 00:00 [accepted]
PHST- 2005/06/14 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/06/14 09:00 [entrez]
AID - S0014-5793(05)00611-3 [pii]
AID - 10.1016/j.febslet.2005.05.013 [doi]
PST - ppublish
SO  - FEBS Lett. 2005 Jun 20;579(16):3437-43. doi: 10.1016/j.febslet.2005.05.013.