PMID- 15951286
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20131121
IS  - 1592-8721 (Electronic)
IS  - 0390-6078 (Linking)
VI  - 90
IP  - 6
DP  - 2005 Jun
TI  - Numerous erythroblasts in maternal blood are impervious to fluorescent in situ 
      hybridization analysis, a feature related to a dense compact nucleus with 
      apoptotic character.
PG  - 740-5
AB  - BACKGROUND AND OBJECTIVES: The analysis by fluorescence in situ hybridization 
      (FISH) of fetal erythroblasts enriched from maternal blood remains an attractive 
      alternative for risk-free prenatal diagnosis of aneuploidies. However, current 
      results are discouraging because of the low levels of sensitivity or the 
      inability to detect fetal erythroblasts by FISH. DESIGN AND METHODS: 
      Erythroblasts were enriched from 35 maternal blood samples by magnetic cell 
      sorting (MACS), identified morphologically following May-Grunwald Giemsa staining 
      and examined by FISH for chromosomes X, Y and 18. RESULTS: We observed that 
      circulating erythroblasts comprised two distinct groups: one was clearly of 
      maternal origin and could be reliably analyzed by FISH, whereas the other, which 
      appeared to be of fetal origin, was largely impervious to FISH analysis. This 
      latter feature seemed to be related to an abnormally dense nucleus with an 
      apoptotic character. Since the oxygen tension in the maternal circulation is 
      higher than that in the fetus, we cultured fetal cord blood erythroblasts in 
      conditions mimicking this difference in oxygen concentrations and found that high 
      oxygen concentrations rapidly induced shrinkage of the erythroblast nucleus, 
      rendering it impervious to FISH analysis. INTERPRETATION AND CONCLUSIONS: Our 
      data show that circulating erythroblasts of presumed fetal origin cannot be 
      reliably analyzed by FISH because of an abnormally dense nucleus. This nuclear 
      phenotype appears to be induced by the higher oxygen tension present in the 
      maternal circulation than in fetal blood.
FAU - Babochkina, Tatiana
AU  - Babochkina T
AD  - University Women's Hospital, Basel, Switzerland.
FAU - Mergenthaler, Susanne
AU  - Mergenthaler S
FAU - De Napoli, Giuseppina
AU  - De Napoli G
FAU - Hristoskova, Sashka
AU  - Hristoskova S
FAU - Tercanli, Sevgi
AU  - Tercanli S
FAU - Holzgreve, Wolfgang
AU  - Holzgreve W
FAU - Hahn, Sinuhe
AU  - Hahn S
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aneuploidy
MH  - *Apoptosis
MH  - Cell Nucleus/*metabolism
MH  - Cell Separation
MH  - Erythroblasts/*cytology
MH  - Female
MH  - Fetal Blood/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Microsatellite Repeats
MH  - Oxygen/metabolism
MH  - Pregnancy
MH  - Prenatal Diagnosis
EDAT- 2005/06/14 09:00
MHDA- 2006/08/11 09:00
CRDT- 2005/06/14 09:00
PHST- 2005/06/14 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2005/06/14 09:00 [entrez]
PST - ppublish
SO  - Haematologica. 2005 Jun;90(6):740-5.