PMID- 15951289
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20061115
IS  - 1592-8721 (Electronic)
IS  - 0390-6078 (Linking)
VI  - 90
IP  - 6
DP  - 2005 Jun
TI  - A new human acute monocytic leukemia cell line SHI-1 with t(6;11)(q27;q23), p53 
      gene alterations and high tumorigenicity in nude mice.
PG  - 766-75
AB  - BACKGROUND AND OBJECTIVES: Human leukemia cell lines are of great value in 
      leukemia research. Thus far 36 leukemia cell lines carrying the 11q23 
      translocation and MLL rearrangements, including two cell lines with 
      t(6;11)(q27;q23) and an MLL-AF6 fusion gene have been described. We have 
      established a new monocytic cell line with t(6;11), designated SHI-1, and herein 
      describe its biological characteristics. DESIGN AND METHODS: Mononuclear cells 
      isolated from the bone marrow of a patient with acute monocytic leukemia 
      (AML-M5b) at relapse were inoculated and passaged by liquid culture. The 
      biological features of the cell line were characterized by morphological assays, 
      flow cytometry, cytogenetic analysis, reverse transcription polymerase chain 
      reaction (RT-PCR), direct sequencing, fluorescence in situ hybridization (FISH), 
      clonogenic culture, quantitative fluorescent PCR, zymography, short tandem 
      repeating sequences-PCR (STR-PCR), multiplex-FISH (M-FISH), and tumorigenic 
      capacity in nude mice. RESULTS: The SHI-1 cell line has been maintained in 
      continuous culture without any external cytokines for three years. The morphology 
      and immunoprofile of the cells show typical features of monocytic lineage. 
      Karyotypic analysis demonstrated a t(6;11)(q27;q23) translocation accompanied by 
      a deletion of 17p, which are the same abnormalities as were seen in the leukemia 
      cells of this patient in relapse. The MLL-AF6 fusion transcript and the loss of 
      one p53 allele were proven by chromosome painting, FISH and RT-PCR analysis in 
      both SHI-1 cells and the primary leukemia cells. A point mutation of ATC-->ACC at 
      codon 195 of exon 6 in another p53 allele was found by direct sequencing of DNA 
      in SHI-1 cells as well as in the primary leukemia cells. Neither Epstein-Barr 
      virus nor mycoplasma was detected in SHI-1 cells. Tumor masses were found in all 
      sixteen mice 9-19 days after subcutaneous injection of SHI-1 cells. DNA 
      fingerprinting confirmed the authenticity of the cell line. INTERPRETATION AND 
      CONCLUSIONS: SHI-1 is a new monocytic leukemia cell line with the t(6;11) 
      translocation, p53 gene alterations, and high tumorigenicity in nude mice. It 
      could be a valuable tool in the study of leukemogenesis.
FAU - Chen, Suning
AU  - Chen S
AD  - First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, 
      Suzhou, China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Zhang, Xueguang
AU  - Zhang X
FAU - Wu, Yafang
AU  - Wu Y
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wang, Yong
AU  - Wang Y
FAU - Ceng, Jiannong
AU  - Ceng J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
MH  - Adult
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Culture Techniques/methods
MH  - *Cell Line, Tumor
MH  - Cell Lineage
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 6
MH  - *Genes, p53
MH  - Humans
MH  - Leukemia, Monocytic, Acute/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Point Mutation
MH  - Recurrence
MH  - *Translocation, Genetic
EDAT- 2005/06/14 09:00
MHDA- 2006/08/11 09:00
CRDT- 2005/06/14 09:00
PHST- 2005/06/14 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2005/06/14 09:00 [entrez]
PST - ppublish
SO  - Haematologica. 2005 Jun;90(6):766-75.