PMID- 15952127
OWN - NLM
STAT- MEDLINE
DCOM- 20090319
LR  - 20050613
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 22
IP  - 3
DP  - 2005 Jun
TI  - [Genomic abnormalities detected by panel fluorescence in situ hybridization in 
      chronic lymphocytic leukaemia].
PG  - 324-6
AB  - OBJECTIVE: To investigate the value of panel fluorescence in situ hybridization 
      for detection of genomic aberrations in chronic lymphocytic leukemia(CLL). 
      METHODS: Five types of fluorescein-labelled DNA probes including centromeric 
      probes for chromosomes 3,12 and 18, and two sequence-specific probes D13S272 for 
      13q14.3 and ATM for 11q23 were used to perform fluorescence in situ hybridization 
      (FISH) assays in 22 patients with CLL. Its results were compared with that of 
      conventional cytogenetic (CC) examination in order to ascertain which method is 
      more sensitive and reliable for the detection of chromosomal and genomic 
      abnormalities in CLL. RESULTS: On CC examination, only 8 cases (36.3%) were found 
      to have chromosomal abnormalities including sole trisomy 12 in 3 cases, 
      simultaneous trisomies 3 and 12 in one case, simultaneous trisomies 3, 12 and 18 
      in one case, translocation between chromosomes 5 and 15 in one case, deletion of 
      13q14.3 in one case, 3q- and 18p+ in one case, 4q+ and 13q- in one case, whereas 
      on panel FISH assay, 15 cases (68.1%) were found to have genomic aberrations 
      including trisomy 3 in 4 cases, trisomy 12 in 6 cases, trisomy 18 in one case, 
      deletion of 13q14.3 in 8 cases, deletion of 11q23 in 6 cases. CONCLUSION: Panel 
      FISH is a useful method for detection of genomic aberration in CLL, if combined 
      with CC, it can obviously enhance the detection rate of chromosomal abnormalities 
      in CLL.
FAU - Li, Qian
AU  - Li Q
AD  - First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Jiang, Hai-yan
AU  - Jiang HY
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Yang, Yan-bo
AU  - Yang YB
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2005/06/14 09:00
MHDA- 2009/03/20 09:00
CRDT- 2005/06/14 09:00
PHST- 2005/06/14 09:00 [pubmed]
PHST- 2009/03/20 09:00 [medline]
PHST- 2005/06/14 09:00 [entrez]
AID - 940622080 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Jun;22(3):324-6.