PMID- 15952381
OWN - NLM
STAT- MEDLINE
DCOM- 20050912
LR  - 20190715
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 39
IP  - 10
DP  - 2005 May 15
TI  - Development of an immunoassay for the determination of polyaromatic hydrocarbons 
      in plasma samples from oiled seabirds.
PG  - 3748-55
AB  - External exposure assessment of oiled seabirds is undertaken by assessment of the 
      percentage oil coverage of the plumage. Nondestructive monitoring of the toxic 
      fraction of petroleum oils and diesels (polyaromatic hydrocarbons, PAHs) which 
      enters the general circulation (internal exposure burden) of oiled seabirds is 
      rarely undertaken. This is because the traditionally used chromatographic methods 
      for plasma PAH analysis require larger sample volumes than those that can be 
      safely collected from smaller species, such as guillemots (Uria aalge). 
      Furthermore, these methods are not a cost-effective or practical approach for 
      analysis of large numbers of birds in a short time period as part of an oil spill 
      response in wildlife rehabilitation centers. This study describes the 
      modification and validation of a commercially available PAH immunoassay (cRaPID 
      PAH) to enable high-throughput, cost-effective, simple, and rapid determination 
      of total PAH concentrations in 50 microL volumes of plasma. The limit of 
      detection of the assay was 0.1 ng/mL as benzo-apyrene (BaP) equivalents with a 
      working range of 0.120 ng/mL. As further validation of the immunoassay, PAHs were 
      determined by GC-MS. GC-MS data were significantly positively correlated with 
      corresponding immunoassay data for the same birds (r2 = 0.976, p < 0.001). The 
      plasma PAH concentrations of 40 oiled guillemots stranded on U.K. shores were 
      determined using the assay to demonstrate its usefulness for biomonitoring 
      studies. The mean ,PAH concentration observed was 1.05 +/- 0.67 ppm (range 
      0.02-2.40 ppm as BaP equivalents). The modifications to the cRaPID PAH kit in 
      this study enable nondestructive, high-throughput, semiquantitative determination 
      of PAH concentrations in plasma samples suitable for exposure assessment of oiled 
      seabirds during oil spill response and rehabilitation.
FAU - Troisi, G M
AU  - Troisi GM
AD  - Environmental Monitoring Unit, School of Engineering & Design, Brunel University, 
      Uxbridge UB8 3PH, United Kingdom. gera.troisi@brunel.ac.uk
FAU - Borjesson, L
AU  - Borjesson L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Antibodies)
RN  - 0 (Petroleum)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Accidents
MH  - Animals
MH  - Antibodies/immunology
MH  - Charadriiformes/blood/metabolism
MH  - Environmental Monitoring/*methods
MH  - Immunoassay/*methods
MH  - *Petroleum
MH  - Polycyclic Aromatic Hydrocarbons/*blood/immunology
MH  - Water Pollutants, Chemical/*blood/immunology
EDAT- 2005/06/15 09:00
MHDA- 2005/09/13 09:00
CRDT- 2005/06/15 09:00
PHST- 2005/06/15 09:00 [pubmed]
PHST- 2005/09/13 09:00 [medline]
PHST- 2005/06/15 09:00 [entrez]
AID - 10.1021/es048935t [doi]
PST - ppublish
SO  - Environ Sci Technol. 2005 May 15;39(10):3748-55. doi: 10.1021/es048935t.