PMID- 15954622
OWN - NLM
STAT- MEDLINE
DCOM- 20050712
LR  - 20061115
IS  - 1373-7503 (Print)
IS  - 1373-7503 (Linking)
VI  - 66
IP  - 3b
DP  - 2001
TI  - Do conventionally and biologically cultivated soils differ in bacterial diversity 
      and community structure?
PG  - 381-8
AB  - The effects of a long-term herbicide treatment on the microbial community of an 
      agricultural soil were investigated. Therefor, molecular techniques were used to 
      evaluate the structure and diversity of the soil microbial community. Eubacterial 
      and group-specific primers for methanotrophs type I and II were used to amplify 
      16S rRNA gene fragments from total soil DNA. These fragments were subsequently 
      separated by denaturing gradient gel electrophoresis (DGGE). The structure of the 
      methanotrophic community was affected by the herbicides as the patterns obtained 
      from a herbicide treated soil (conventional) clustered separately from the 
      control soil (biological). It seems that group-specific PCR followed by DGGE is a 
      very powerful and sensitive technique to differentiate fields, which have 
      received a herbicide treatment from those who did not. The diversity of the 
      methanotrophic community was quantified by calculating the Shannon-Weaver index 
      of biodiversity. The type I methanotrophs showed a significant decreased 
      biodiversity due to the herbicide treatment but the diversity of the 
      methanotrophs type II was slightly higher in the herbicide treated soil.
FAU - Seghers, D
AU  - Seghers D
AD  - Laboratory of Microbial Ecology and Technology, Ghent University, Coupure Links 
      653, B-9000 Gemt, Belgium.
FAU - Reheul, D
AU  - Reheul D
FAU - Bulcke, R
AU  - Bulcke R
FAU - Verstraete, W
AU  - Verstraete W
FAU - Top, E M
AU  - Top EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Belgium
TA  - Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet
JT  - Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en 
      Toegepaste Biologische Wetenschappen)
JID - 100967625
RN  - 0 (DNA, Bacterial)
RN  - 0 (Herbicides)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*drug effects/*genetics/isolation & purification/metabolism
MH  - Biodegradation, Environmental
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - Colony Count, Microbial
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Herbicides/*pharmacology
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Sensitivity and Specificity
MH  - *Soil Microbiology
EDAT- 2005/06/16 09:00
MHDA- 2005/07/13 09:00
CRDT- 2005/06/16 09:00
PHST- 2005/06/16 09:00 [pubmed]
PHST- 2005/07/13 09:00 [medline]
PHST- 2005/06/16 09:00 [entrez]
PST - ppublish
SO  - Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66(3b):381-8.